项智锋;李艳;王清华;

• 1:河南科技学院动物科学学院
• 2:河南科技学院动物科学学院
• 3:河南科技学院动物科学学院 河南新乡453003
• 4:河南新乡453003

摘要(Abstract)：

猪卵母细胞体外成熟(IVM)为猪胚胎的体外生产及相关胚胎工程技术如胚胎细胞和体细胞核移植、转基因和性别控制提供了廉价原料,是现代胚胎工程技术的研究热点之一。多年来科研人员为了进一步提高卵母细胞体外培养成熟率以及更深入地了解卵母细胞成熟机制,对影响卵母细胞体外成熟的因素进行了大量的研究。综述了影响猪卵母细胞体外成熟的相关因素。

关键词(KeyWords)： 体外成熟;卵母细胞;胚胎工程

Abstract:

Keywords:

基金项目(Foundation): 河南科技学院引进人才基金项目

作者(Author): 项智锋;李艳;王清华;

Email:

DOI: 10.13989/j.cnki.0517-6611.2006.21.058

参考文献(References)：

• [1]HYUN H,LEE G S,KIM D Y,et al.Effects of maturation media and oocytederived from sows or gilts on the development of cloned pig embryos[J].Theri-ogenology,2003,38(5):15-16.
• [2]郭宪,陈学进,叶荣,等.牛卵巢卵母细胞体外成熟的研究[J].甘肃农业大学学报,2004(5):503-507.
• [3]WALTERS E M,GRAVES C N.Transportation and storage effects on porcineovaries[J].J Anim Sci,1998,76:69-72.
• [4]ABEYDEERA L R.In vitro production of embryos in swine[J].Theriogenolo-gy,2002,57:252-273.
• [5]陈晓宇,刘东,李青旺,等.猪卵巢卵母细胞的收集和体外成熟培养[J].云南畜牧兽医,2002(4):39-41.
• [6]YOONKW,SHINTY,PARK J I,et al.Development of porcine oocytes frompreovulatory follicles of different sizes after maturation in media supplementedwith follilcular fluids[J].Reprod Fertil Dev,2000,12:133-139.
• [7]韩毅冰,施维民,秦鹏春.猪不同直径卵泡卵母细胞体外培养的成熟能力[J].中国兽医学报,2002(20):183-187.
• [8]LUCAS X,MARTINEZ E A,ROCA J,et al.Influence of follicle size on thepenetrability of immature pig oocytes for in vitro penetration assay[J].Theri-ogenology,2003,60:659-667.
• [9]李丽立,王芳.现代生物技术与畜牧业[M].北京:科学出版社,2002:10-12.
• [10]SHIMADA M,KAWAN N,TERADAT.Delay of nuclear maturation and re-action in developmental competence of pig oocytes after mineral overlay of invitro maturation media[J].Reproduction,2002,124:557-564.
• [11]王海,曾申明,朱士恩,等.培养介质、卵丘细胞和卵泡直径对猪卵母细胞体外成熟的影响[J].中国畜牧杂志,2002,38(5):15-16.
• [12]叶华虎,魏泓,陈紧顾.猪卵母细胞在不同培养液中成熟后的发育能力[J].第三军医大学学报,2003,25(6):511-513.
• [13]蔡令波,王锋.猪卵母细胞体外成熟与冷冻保存的初步研究[J].南京农业大学学报,2002,25(4):116-118.
• [14]MARGOT ALVES,CHARLES GRAVES.Involvement of steroid hormones onin vitro maturation of pig oocytes[J].Theriogenology,2002,57:811-821.
• [15]黄志坚,黄依明.牛体外受精技术研究进展[J].福建畜牧兽医,2002(1):56-58.
• [16]JI WU,BEN JAMIN,DONGLAS CARREA.In vitro growth,maturation,fer-tilization and embryonic development of oocytes from preantral follicles[J].Bi-ol Reprod,2001,64:375-381.
• [17]YAMANCHI N,NAGIA T.Male pronuclear formation on in denuded porcineoocyte after in vitro maturation in the presence of cysteamin[J].Biol Reprod,1999,61:828-833.
• [18]YASHIDA M,ISHIZAKI Y,KAWAGISHI H,et al.Effects of pig follicularfluid on maturation of pig oocytes in vitro and on their subsequent fertilizingand developmental capacity in vitro[J].Journal of Reproduction and Fertility,1992,95(2):481-488.
• [19]GEORGIOS VATZIAS,DANIEL HAGEN.Effects of porcine follicular fluidand oviduct conditioned madia on maturation and fertilization of porcine oocytesin vitro[J].Biol Reprod,1999,28:58-66.
• [20]苏有强,夏国良,陈勇,等.HX对猪卵母细胞体外自发成熟抑制作用的研究[J].畜牧兽医学报,2000,31(1):22-27.
• [21]HYTTEL P,GREVE Y.Changes in cortical granules during porcine oocytematuration[J].Gamete Res,1999,11(3):330-332.
• [22]ABEYDEERA L R,WANG WH,CONTLEYT C,et al.Presence of-βmer-captothanol can increase the glutathione content of pig oocyte after in vitro fer-tilization:relevance to intracellular glutathione[J].Bilo Reprod,1998,58:213-218.
• [23]HIDEKI TATEMOTO,KEISUKE OOTAKI,KOJI SHIGETA,et al.Enhance-ment of developmental competence after in vitro fertilization of porcine oocytesby treated with AA-2G during in vitro maturation[J].Biol Reprod,2001,65:1 800-1 806.