体外培养刺五加变异的RAPD分析Analysis of the Variation of Eleutherococcus senticosns Cultuered in Vitro with RAPD
邢朝斌;王明艳;马百成;刘岩;罗玲;
摘要(Abstract)：
以刺五加的成熟种子为材料,通过离体培养诱导产生愈伤组织,利用RAPD分子标记方法,在DNA水平上分析愈伤组织与其来源植株间的遗传差异。从40个引物(10碱基的寡核苷酸)中筛选出6个引物,对它们的PCR扩增结果进行分析。结果表明,刺五加愈伤组织与其来源植株相比出现了DNA水平的变异。
关键词(KeyWords)： 刺五加;愈伤组织;RAPD
基金项目(Foundation):
作者(Author): 邢朝斌;王明艳;马百成;刘岩;罗玲;
Email:
DOI: 10.13989/j.cnki.0517-6611.2006.21.019
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