体外培养刺五加变异的RAPD分析Analysis of the Variation of Eleutherococcus senticosns Cultuered in Vitro with RAPD
邢朝斌;王明艳;马百成;刘岩;罗玲;
摘要(Abstract)：
以刺五加的成熟种子为材料,通过离体培养诱导产生愈伤组织,利用RAPD分子标记方法,在DNA水平上分析愈伤组织与其来源植株间的遗传差异。从40个引物(10碱基的寡核苷酸)中筛选出6个引物,对它们的PCR扩增结果进行分析。结果表明,刺五加愈伤组织与其来源植株相比出现了DNA水平的变异。
关键词(KeyWords)： 刺五加;愈伤组织;RAPD
基金项目(Foundation):
作者(Author): 邢朝斌;王明艳;马百成;刘岩;罗玲;
Email:
DOI:
参考文献(References)：
- [1]邢朝斌,沈海龙,赵丽娜,等.刺五加的体细胞胚胎发生研究[J].中草药,2006,37(5):769-772.
- [2]CHOI Y E,YANG D C,YOON E S.Rapid propagation ofEleutherococcus senti-cosusvia direct somatic embryogenesis from explants of seedlings[J].Plant Cell,Tissue and Organ Culture,1999,58:93-97.
- [3]CHOI Y E,KIM J W,YOON E S.High frequency of plant production via somaticembryogensis from callus or cell suspension cultures inEleutherococcus sentico-sus[J].Annals of Botany,1999,83:309-314.
- [4]BROWNPTH,LANG F D,KRANZ E.Analysis of single protoplasts and regen-erated plants by PCR and RAPD technology[J].Mol Gen Genet,1993,237:211-317.
- [5]GUDWIN L D,SANGDUNE N K,UNANUVATCH R,et al.RAPD poly mor-phisns among variant and phenotypically normal rice somaclonal progenies[J].Plant Cell Rep,1997,16:320-324.
- [6]邢朝斌,沈海龙,王明艳,等.刺五加的无菌发芽研究[J].种子,2005,24(12):30-33.
- [7]付杨,陈凤清,孙冬雪,等.大叶柴胡愈伤组织继代培养及其RAPD分析[J].东北师范大学学报:自然科学版,2005,37(2):90-92.
- [8]FLUMINHAN A,DE A,PERECIN MLR,et al.Evidence for heterochromatin in-volvement in chromosome breakage in maize callus culture[J].Ann Bot,1996,78:73-82.