香蕉条斑病毒CP区基因的克隆及其在大肠杆菌中的融合表达Cloning of Coat Protein Gene of Banana Streak Virus and its Fusion Expression in Escherichia coli
邱世明;庄军;刘志昕;
摘要(Abstract)：
依据锌指结构在BSVCP上的可能位置合成了2条引物,通过PCR扩增得到长约640 bp的目的片段;将目的片段与质粒pET28 b(+)连接,构建了含BSVCP区基因的融合蛋白原核表达载体pET28b-BSVCP;克隆测序以确定其阅读编码框的正确性,然后用正确的重组质粒转化大肠杆菌Rosetta(DE3),并诱导表达;经IPTG诱导后,出现一特异的约30 kDa融合蛋白质表达条带。
关键词(KeyWords)： 香蕉条斑病毒;外壳蛋白;原核表达
基金项目(Foundation): 国家自然科学基金资助项目(30660100);; 海南省教育厅高等学校科研资助项目(Hjkj200505)
作者(Author): 邱世明;庄军;刘志昕;
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参考文献(References)：
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