樊俊;朱道立;

• 1:南通大学生命科学学院

摘要(Abstract)：

[目的]研究成年大鼠快肌内侧腓肠肌与慢肌比目鱼肌内各型肌纤维的分布及构成差异。[方法]应用RT-PCR方法分析4型肌纤维的肌球蛋白重链异构体基因表达差异,并以肌动球蛋白腺苷三磷酸酶组织化学染色分析4型肌纤维构成比例。[结果]通过RT-PCR扩增所得的条带,在内侧腓肠肌内MHCⅠ、MHCⅡx、MHCⅡa、MHCⅡb基因表达均有分布,而在比目鱼肌中未发现有Ⅱb型基因表达。4型或3型肌纤维均呈交错式镶嵌型分布。[结论]大鼠内侧腓肠肌中ⅡA型肌纤维占很大比例,而在比目鱼肌中I型肌纤维最多,其中缺少ⅡB型肌纤维。

关键词(KeyWords)： 肌纤维;肌球蛋白重链异构体;聚合酶链式反应;肌动球蛋白腺苷三磷酸酶;大鼠

Abstract:

Keywords:

基金项目(Foundation): 南通大学第3批大学生课外学术科技作品立项课题(加16)

作者(Author): 樊俊;朱道立;

Email:

DOI: 10.13989/j.cnki.0517-6611.2008.30.038

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