周索;王云;庞振凌;

• 1:南阳师范学院生物系
• 2:南阳师范学院生物系
• 3:南阳师范学院生物系 河南南阳473061
• 4:河南南阳473061

摘要(Abstract)：

采用不用浓度ABA处理各种基因型的拟南芥种子,结果表明:拟南芥abi1和abi2是ABA信号的负调节者;异三聚体G蛋白α亚基gpa1基因超表达株系和T-DNA插入突变体在种子萌发和幼苗发育中对ABA不敏感;相对于野生型来说,a/c、a/g表现出相对的ABA不敏感性,表明在ABA抑制的种子萌发和幼苗发育过程中gap1可能位于abi1上游。

关键词(KeyWords)： 拟南芥;脱落酸;种子萌发;幼苗发育

Abstract:

Keywords:

基金项目(Foundation): 南阳师范学院资助。

作者(Author): 周索;王云;庞振凌;

Email:

DOI:

参考文献(References)：

• [1]FINKELSTEIN R R,GAM PALA S S L,ROCK C D.Abscisic acidsignaling in seeds and seedlings[J].Plant Cell,2002(Sup):15-45.
• [2]GOSTI F,BEAUDOIN N,SERIZET C,et al.ABI1protein phosphatase2C is a negative regulator of abscisic acid signaling[J].Plant Cell,1999,11:1897-1909.
• [3]LEUNG J,M ERLOT S,GIRAUDAT J.The Arabidopsis ABSCISIC ACID-INSENSITIVE2(ABI2)and ABI1genes encode hom ologous protein phosphatases2C involved in abscisic acid signal transduction[J].Plant Cell,1997,9:759-771.
• [4]M ERLOT S,GOSTI F,GUERRIER D,et al.The ABI1and ABI2protein phosphatase2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway[J].Plant J,2001,25:295-303.
• [5]M URATA Y,PEI Z M,M ORI I C,et al.Abscisic acid activation of plasm a m em brane Ca2+channels in guard cells requires cytosolic NAD(P)H and is differentially disrupted upstream and downstream of reactive oxygen species production in abi1-1and abi2-1protein phosphatase2C m utants[J].Plant Cell,2001,13:2513-1523.
• [6]JONES A M,ASSM ANN S M.Plants:the latest model system for G-protein research[J].EM BO J,2004,5:572-578.
• [7]M A H,YANOFSKY M F,M EYEROW ITZ E M.M olecular cloning and characterization of GPA1,a G proteinα-subunit gene from Arabidopsis thaliana[J].Proc Natl Acad Sci USA,1990,87:3821-3825.
• [8]ULLAH H,CH EN J G,W ANG S,et al.Role of G protein in regulation of Arabidopsis seed germ ination.Plant Physiol,2002,129:897-907.
• [9]OKAM OTO H,M ATSUI M.Overexpression of the H eterotrim eric G-Proteinα-Subunit Enhances Phytochrom e-M ediated Inhibition of Hypocotyl Elongation in Arabidopsis[J].The Plant Cell,2001,13:1639-1651.