李永丽;史洪中;陈利军;周洲;

• 1:信阳农业高等专科学校农业科学系
• 2:信阳农业高等专科学校农业科学系
• 3:信阳农业高等专科学校农业科学系
• 4:中国林业科学研究院林业研究所生物技术室 河南信阳464000

摘要(Abstract)：

转基因技术为培育抗黄矮病小麦提供了一条有力的途径,综述国内外在小麦抗BYDV的转基因研究进展。探讨目前主要基于BYDV自身的外壳蛋白基因、复制酶基因、运动蛋白基因以及蚜虫传毒相关蛋白基因进行的抗BYDV小麦转基因研究现状和成果,并对未来培育抗黄矮病小麦研究进行展望。

关键词(KeyWords)： 转基因小麦;大麦黄矮病毒;外壳蛋白;复制酶;运动蛋白;传毒蛋白

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 李永丽;史洪中;陈利军;周洲;

Email:

DOI: 10.13989/j.cnki.0517-6611.2006.21.002

参考文献(References)：

• [1]MILLER AW,RASOCHOV.áL.Barley yellow dwarf viruses[J].Annu Rev Phy-topathol,1997,35:167-90.
• [2]MAKKOUK K M,JEALINSKI H.The“yellow plague of cereals,barley yellowdwarf virus[C]//World perspectives on barely yellow dwarf.CIMMYT MEXI-CO,D F,MEXICO,1987:1-6..
• [3]韩志勇,赵章杏,朱睦元.大麦黄矮病毒分子生物学的研究进展[J].中国病毒学,1998,13(4):292-299.
• [4]张淑香,周广和.一种由麦二叉蚜、禾缢管蚜非专化性传毒的小麦黄矮病毒株系鉴定[J].植物病理学报,1987,17(2):102-105.
• [5]成卓敏,吴茂森,夏光敏,等.应用基因枪法获得抗大麦黄矮病毒转基因小麦[J].植物病理学报,2000,30(2):116-120.
• [6]POWELL P A,STARK D M,SANDERS P R,et al.Protection against tobaccomosaic virus in transgenic plants that express tobacco mosaic virus antisenseRNA[J].PNAS,1989,86:6 949-6 952.
• [7]CLARK W G,FITCHEN J,NEJIDAT A,et al.Studies of coat protein-mediatedresistance to tobacco mosaic virus(TMV).II.Challenge by a mutant with al-tered virion surface does not overcome resistance conferred by TMV coat protein[J].J Gen Virol,1995,76:2 613-2 617.
• [8]BEACHY R N,LOESCH F S,TUMER N E.Coat protein mediated resistance a-gainst virus infection[J].Annual Review of Phytopathology,1990,28:451-474.
• [9]MURRY L E,ELLIOT L G,CAPITANT S A,et al.Transgenic corn plants ex-pressing MDMV strain B coat protein are resistant to mixed infections of maizedwarf mosaic virus and maize chlorotic mottle virus[J].Bio Technology,1993,11:1 559-1 564.
• [10]MCCARTHY P L,HANSEN J,SHIEL P J,et al.Cp-mediated resistance towheat streak mosaic and barley yellow dwarf viruses in soft white winter wheats[C].Jerusalem:International Congress of Virology,1996:184.
• [11]MCGRATH P F,VINCENT J R,LEI C H,et al.Coat protein mediated resis-tance to isolates of barley yellow dwarf in oats and barley[J].European Journalof Plant Pathology,1997,103:695-710.
• [12]成卓敏,何小源,陈彩层,等.大麦黄矮病毒外壳蛋白基因合成及用花粉管途径获得小麦转基因植株[J].自然科学进展,1993,3(6):560-564.
• [13]成卓敏,吴茂森,夏光敏,等.应用基因枪法获得抗大麦黄矮病毒转基因小麦[J].植物病理学报,2000,30(2):115-120.
• [14]周广和,张淑香,钱幼亭.小麦黄矮病毒4种株系鉴定与应用[J].中国农业科学,1987,20(4):7-12.
• [15]贾力,吴茂森,张文蔚,等.大麦黄矮病毒单双价外壳蛋白基因植物表达载体构建及小麦遗传转化[J].农业生物技术学报,2001,9(1):23-27.
• [16]GOLEMBOSKI D B,LOMONOSSOFF G P,ZAITLINM.Plants transformed witha tobacco mosaic virus nonstructural gene sequence are resistant to the virus[J].Proc Natl Acad Sci USA,1990,87:6 311-6 315.
• [17]BAULCOMBE D.Replicase-mediated resistance:a novel type of virus resistancein transgenic plants[J].Trents in Microbiology,1994,2(2):60-63.
• [18]ANDERSON J M,PALUKAITIS P.A defective replicase gene induces resistanceto cucumber mosaic virus in transgenic tobacco plants[J].Proc Nal Acad SciUSA,1992,89:8 759-8 763.
• [19]RUBINO L,LUPO R,RUSSO M.Resistance to cymbidium rings pot tombusvirus infection in transgenic Nicotiana benthamiana plants expressing a fulllength viral replicase gene[J].Molecular Plant Microbe Interaction,1993,6:729-734.
• [20]BRAUN C J,HEMENWAY C L.Expression of amino terminal portions or fulllength viral replicase genes in transgenic plants confers resistance to potatovirus X infection[J].The Plant Cell,1992,4:735-744.
• [21]MACFARLANE S A,DAVIES J W.Plants transformed with a region of the 201-kilodalton replicase gene from pea early browning virus RNA 1 are resistant tovirus infection[J].Proc Natl Acad Sci USA,1992,89:5 829-5 833.
• [22]项瑜,杨兰英,彭学贤,等.改造的马铃薯Y病毒复制酶基因介导高度抗病性[J].生物工程学报,1996,12(3):258-265.
• [23]MILLER W A,RASOCHOVA L.Barley yellow dwarf viruses[J].Annu RevPhytopathol,1997,35:167-190.
• [24]KOEV G,MOHAN B R,DINESH-KUMAR S P,et al.Extreme reduction of dis-ease in oats transformed with the 5’half of the barley yellow dwarf virus-PAVgenome[J].Phytopathology,1998,88:1 013-1 019.
• [25]WANG M,ABBOTT D C,WATERHOUSE P M.A single copy of a virus-de-rived transgene encoding hairpin RNA gives immunity to barley yellow dwarfvirus[J].Molecular Plant Pathology,2000,1:347-356.
• [26]ABBOTT D,WANG M B,WATERHOUSE P.A single copy of a virus-derivedtransgene encoding hairpin RNA confers BYDV immunity[C]//HENRY M,MCNAB A.Barley Yellow Dwarf Disease:Recent Advances and Future Strate-gies.CIMMYT,Texcoco,Mexico,2002:22-26.
• [27]DUPRéP,HENRY M,POSADAS G,et al.Genetically engineering wheat forbarley yellow dwarf virus resistance[C]//HENRY M,MCNAB A.Barley Yel-low Dwarf Disease:Recent Advances and Future Strategies.CIMMYT,Texcoco,Mexico,2002:27-28.
• [28]田苗英,吴茂森,陈彩层,等.大麦黄矮病毒缺失复制酶基因植物表达载体的构建及转基因小麦的获得[J].中国农业科学,1999,32(5):49-54.
• [29]吴茂森.大麦黄矮病毒GPV株系复制酶基因介导的抗病毒转基因小麦的研究[D].北京:中国农业科学院,2000.
• [30]吴茂森,田苗英,陈彩层,等.通过花粉管通道途径将BYDV-GPV株系缺失复制酶基因导入小麦[J].农业生物技术学报,2000,8(2):125-127.
• [31]刘太国.BYDV-GPV株系正向串联体结构复制酶基因介导的转基因小麦的研究[D].北京:中国农业科学院,2003.
• [32]刘太国,吴茂森,张文蔚,等.花粉管通道法介导的抗大麦黄矮病毒转基因小麦研究[J].植物保护,2005,31(4):32-35.
• [33]晋治波.大麦黄矮病毒GAV基因组全序列分析及运动蛋白基因介导的抗病毒小麦的研究[D].北京:中国农业科学院,2003.
• [34]COOPER B,LAPIDOTM,HEICK J A,et al.A defective movement protein ofTMV in transgenic plants confers resistance to multiple viruses whereas thefunction analog increases susceptibility[J].Virology,1995,206:307-313..
• [35]LAPIDOT M,GAFNY R,DING B,et al.A dysfunctional movement protein oftobacco mosaic virus that partially modifies the plasmodesmata and limits virusspread in transgenic plants[J].The Plant Journal,1993,4:959-970.
• [36]MALYSHENKO S I,KONDAKOVA O A,NAZAROVA J V,et al.Reduction oftobacco mosaic virus accumulation in transgenic plants producing non-functionalviral transport proteins[J].Gen Virol,1993,74:1 149-1 156.
• [37]ZIEGLER-GRAFF V,GUILFORD P J,BAULCOMBE D C.Tobacco rattle virusRNA-1.29K gene product potentiates viral movement and also affects symptominduction in tobacco[J].Virology,1991,182:145-155..
• [38]王锡锋,周广和.大麦黄矮病毒介体麦二叉蚜和麦长管蚜体内传毒相关蛋白的确定[J].科学通报,2003,48(15):1 671-1 675.
• [39]SINGH R P.Genetic associaton of gene Bdv1 for tolerance to barley yellowdwarf viruswith gene Lr34 and Yr18 for adult plant resistance torusts in bread-wheat[J].Plant Disease,1993,77(11):1 103-1 106.
• [40]KING B,PALTRIDGE N,COLLINS N,et al.A map-based approach towardscloning the Yd2 resistance gene in barley[C]//HENRY M,MCNAB A.BarleyYellow Dwarf Disease:Recent Advances and Future Strategies.CIMMYT,Tex-coco,Mexico,2002:75-77.
• [41]XIN Z Y,XU H J,CHEN X,et al.Development of common wheat germplasmresistant to barley yellow dwarf virus by biotechnology[J].Science in China(Series B),1991,34:1 055-1 062.
• [42]刘艳,钱幼亭,赵茂林,等.多枝赖草及其转育后代对大麦黄矮病毒PAV和GAV株系的抗性研究[J].植物病理学报,2002,32(3):247-251.
• [43]谢皓,陈孝,张增艳,等.抗黄矮病小麦新品系YW243的选育和细胞分子生物学鉴定[J].作物学报,2000,26(6):687-691.
• [44]BANKS P M,LARKIN P J,BARIANA H S,et al.The use of cell culture forsubchromosomal introgressions of barley yellow dwarf virus resistance fromThinopyrum intermedium to wheat[J]Genome,1995,38:395-405.
• [45]HOHMANN U,BADAEVA K,BUSCH W,et al.Molecular cytogenetic analysisof Agropyron chromatin specifying resistance to barley yellow dwarf virus inwheat[J].Genome,1996,39:336-34.
• [46]张增艳,辛志勇,马有志用分子标记定位源于中间偃麦草的小麦抗黄矮病基因[J].中国科学(C辑),1999,29(4):414-419.