超高效液相色谱-三重四级杆质谱同时检测苦荞中10种真菌毒素Simultaneous Determination of 10 Mycotoxins in Buckwheat with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
唐振涛;于刚;刘菲;俞凌云;薛康;
摘要(Abstract):
[目的]建立QuEChERS-超高效液相色谱-串联质谱法同时检测苦荞样品中10种真菌毒素。[方法]苦荞样品经水和乙腈提取后,采用QuEChERS方法净化。通过超高效液相色谱-三重四级杆质谱在多反应监测模式(MRM)下进行目标真菌毒素的分析。试验对样品前处理、仪器分析条件进行优化;考察方法的基质效应、检出限、精密度、回收率等参数。[结果]根据基质效应考察结果,50%的目标真菌毒素具有显著的信号抑制/增强效应。为了补偿基质效应的影响,选用基质配标法进行目标真菌毒素的分析。方法的线性范围为0.5~50.0μg/L(对于黄曲霉毒素B_2、G_2,线性范围为0.125~12.500μg/L),检出限和定量限分别低至0.011和0.040μg/L,回收率为89.70%~105.83%,RSD<9%(n=6)。[结论]建立的方法简便、可靠,具有较好的灵敏度和准确性,能有效用于苦荞样品中多组分真菌毒素的检测。
关键词(KeyWords): 苦荞;多组分真菌毒素;QuEChERS;超高效液相色谱-三重四级杆质谱
基金项目(Foundation): 四川省科技项目(2018JY0427)
作者(Author): 唐振涛;于刚;刘菲;俞凌云;薛康;
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DOI:
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