姚清国;李小兵;韩爱云;

• 1:石家庄学院化工学院

摘要(Abstract)：

[目的]在大肠杆菌中诱导表达水稻蛋白OsOle1。[方法]RT-PCR克隆水稻基因OsOle1,构建原核表达载体pGEX-2T-OsOle1-GST,在大肠杆菌BL21中诱导表达该蛋白,并采用GST凝胶亲和层析进行纯化。[结果]水稻蛋白OsOle1具有典型的Olee1蛋白家族的保守域,N端1～24位氨基酸为预测的信号肽序列。成功地将水稻蛋白OsOle1在大肠杆菌中进行了诱导表达,并用GST亲和层析进行了纯化。[结论]该研究为以后进一步研究水稻蛋白OsOle1的生理功能奠定了基础。

关键词(KeyWords)： 水稻;OsOle1;诱导表达;亲和层析

Abstract:

Keywords:

基金项目(Foundation): 石家庄市科学技术研究与发展课题(10120803);; 石家庄学院科研启动基金(2007012)

作者(Author): 姚清国;李小兵;韩爱云;

Email:

DOI: 10.13989/j.cnki.0517-6611.2011.19.192

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