余兴;丁伟;王蕾;贾兵;朱立武;

• 1:安徽省地质实验研究所
• 2:安徽农业大学园艺学院

摘要(Abstract)：

[目的]克隆杜梨硝酸盐高亲和性转运酶NRT2基因。[方法]以杜梨叶片为试验材料,采用CTAB法提取总RNA,反转录成c DNA,运用3'-RACE和5'-RACE技术,克隆获得杜梨硝酸盐转运酶NRT2基因的全长,并运用生物信息学技术对硝酸盐转运酶NRT2基因进行了分析。[结果]杜梨硝酸盐转运NRT2基因c DNA全长为1 422 bp,可编码473个氨基酸,分子式为C2346H3631N589O637S26,相对分子质量为51 112.7,等电点(p I)为7.03。[结论]该试验为进一步研究梨氮素代谢提供了基础资料。

关键词(KeyWords)： 杜梨;硝酸盐转运酶基因;克隆

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 余兴;丁伟;王蕾;贾兵;朱立武;

Email:

DOI: 10.13989/j.cnki.0517-6611.2017.02.053

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