改良大豆胚尖再生体系的研究Study on the Improvement of Embryonic Tip Regeneration System of Soybean
姚燕;张学杰;张慧;王绍明;
摘要(Abstract)：
为了研究利用Thidiazuron(TDZ)建立大豆胚尖高效再生体系的可行性。跃进10号的成熟种子浸泡16 h后,收集胚尖转入添加了不同浓度的TDZ芽诱导培养基中,4周后计算出芽率,并对拔高培养基和生根培养基进行优化。TDZ浓度为0.08 mg/L时,出芽率最高,达92.6%,平均每个外植体产生的丛生芽的数目也最多。适宜大豆胚尖的芽诱导分化培养基为MS+0.08 mg/L TDZ,拔高培养基为MS+2.0 mg/L KT+0.2 mg/L NNN,生根培养基为1/2MS+1.0 mg/L IBA。利用TDZ建立大豆胚尖高效再生体系是可行的,提高了丛生芽的分化率,为快繁和提高遗传转化效率奠定了坚实的基础。
关键词(KeyWords)： 大豆;胚尖再生体系;Thidiazuron(TDZ)
基金项目(Foundation):
作者(Author): 姚燕;张学杰;张慧;王绍明;
Email:
参考文献(References)：
- [1]HINCHEE M A W,CONNR WARD D V,NEWELL C A,et al.Production oftransgenic soybean plants using Agrobacterium-mediated DNA transfer[J].Bio/Technology,1988(6):915-922.
- [2]MCCABE D E,SWAIN W F,MARTINELL B J,et al.Stable transformation ofsoybean(Glycine max)by particle acceleration[J].Bio/Technology,1988(6):923-926.
- [3]TRICK H N,DINKINS R D,SANTARN E R,et al.Recent advances in soybeantransformation[J].Plant Tiss Cult Biotechnol,1997(3):9-26.
- [4]SHAN Z,RAEMAKERS K,TZITZIKAS E N,et al.Development of a highly effi-cient,repetitive system of organogenesis in soybean(Glycine max(L.)Merr)[J].Plant Cell Rep,2005,24:507-512.
- [5]LIUH,YANG C,WEI Z.EfficientAgrobacteriu mtumefaciensmediated transfor-mation of soybeans using an embryonic tip regeneration system[J].Planta,2004,219:1042-1049.
- [6]邓向阳,卫志明.大豆转化技术[J].植物生理学通讯,1998,34:381-387
- [7]TZITZIKAS E N,BERGERVOETM,RAEMAKERS K,et al.Regeneration of Pea(Pisum sativumL.)by a cyclic organogenic system[J].Plant Cell Rep,2004,23:453-461.
- [8]李明春,蔡易,赵桂兰,等.改良大豆子叶节再生体系的研究[J].作物学报,2006(32):223-227.