改良大豆胚尖再生体系的研究Study on the Improvement of Embryonic Tip Regeneration System of Soybean
姚燕;张学杰;张慧;王绍明;
摘要(Abstract)：
为了研究利用Thidiazuron(TDZ)建立大豆胚尖高效再生体系的可行性。跃进10号的成熟种子浸泡16 h后,收集胚尖转入添加了不同浓度的TDZ芽诱导培养基中,4周后计算出芽率,并对拔高培养基和生根培养基进行优化。TDZ浓度为0.08 mg/L时,出芽率最高,达92.6%,平均每个外植体产生的丛生芽的数目也最多。适宜大豆胚尖的芽诱导分化培养基为MS+0.08 mg/L TDZ,拔高培养基为MS+2.0 mg/L KT+0.2 mg/L NNN,生根培养基为1/2MS+1.0 mg/L IBA。利用TDZ建立大豆胚尖高效再生体系是可行的,提高了丛生芽的分化率,为快繁和提高遗传转化效率奠定了坚实的基础。
关键词(KeyWords)： 大豆;胚尖再生体系;Thidiazuron(TDZ)
基金项目(Foundation):
作者(Author): 姚燕;张学杰;张慧;王绍明;
Email:
DOI: 10.13989/j.cnki.0517-6611.2007.21.073
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