王雅楠;王军军;徐大勇;蒋伶活;

• 1:江南大学生物工程学院国家工程实验室

摘要(Abstract)：

[目的]研究Ca FIG1基因除参与有性生殖过程外,是否还参与细胞钙稳态等其他功能,对Ca FIG1进行基因敲除。[方法]采用2种方法分别敲除Ca FIG1的2个等位基因,一种通过PCR扩增HIS1敲除盒,另一种通过克隆的手段构建URA3敲除盒。[结果]从白念珠菌RM1000中成功敲除了Ca FIG1双等位基因。[结论]该敲除策略同样适用于其他基因的敲除,提高了基因敲除的效率。

关键词(KeyWords)： 白念珠菌;CaFIG1;基因敲除

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81371784);; 江南大学自主研究计划重点项目(JUSRP51313B)

作者(Author): 王雅楠;王军军;徐大勇;蒋伶活;

Email:

DOI: 10.13989/j.cnki.0517-6611.2015.17.048

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