邹烨;王彩霞;赵婷;周叶;吴向阳;赵江丽;仰榴青;

• 1:江苏大学化学化工学院
• 2:江苏大学食品与生物工程学院
• 3:江苏大学药学院

摘要(Abstract)：

[目的]优化花生根茎叶中白藜芦醇超声法提取的工艺条件。[方法]以花生根、茎、叶为原料,采用超声法对白藜芦醇的提取工艺进行研究,通过单因素试验和正交试验确定提取的最佳工艺条件,并通过HPLC法进行分析。[结果]超声提取法提取花生根、茎、叶中白藜芦醇的最佳工艺为:超声频率为15.33 kHz,占空比为1∶2,提取时间为6 min,料液比为1∶25,乙醇浓度为70%,在最佳提取条件下测得白藜芦醇的提取量为885.96μg/g。[结论]该研究得到超声提取法提取花生根、茎、叶中白藜芦醇的最佳提取工艺,为高效提取花生根茎叶中的白藜芦醇提供了新途径。

关键词(KeyWords)： 花生根茎叶;白藜芦醇;超声提取

Abstract:

Keywords:

基金项目(Foundation): 镇江市农业科技计划项目(NY2008044)

作者(Author): 邹烨;王彩霞;赵婷;周叶;吴向阳;赵江丽;仰榴青;

Email:

DOI: 10.13989/j.cnki.0517-6611.2011.04.119

参考文献(References)：

• [1]JANGMCAI LN,UDEANI G O,et al.Cancer chemopreventive activity ofresveratrol,a natural product derived from grapes[J].Science,1997,275(5297):218-220.
• [2]GEHMB D,MCANDREWSJ M,CHIENPY,etal.Resveratrol,a polyphe-nolic compound found in grapes and wines,is agonist for the estrogen re-ceptor[J].Proc,Natl Acad.Sce USA,1997,94:14138-14143.
• [3]AHAMD N,ADHAMI VM,AFAQF,etal.Resveratrol cause WAF-1/p21-mediated G(1)-phase arrestofcell cycleand induction ofapoptosis in hu-man epidermoid carcinoma A431cells[J].Clin Cancer Res,2001,7(5):1466-1473.
• [4]HOLIANO,WALTER R J.Resveratrol inhibits the proliferation of normalhuman keratinocytes in vitro[J].Cell Biochem,2001,81(36):55-62.
• [5]CADENAS S,BARJA G.Resveratrol,melatonin,vitamin E,and PBN pro-tect against renal oxidative DNAdamageinduced by the kidney carcinogenKBrO3[J].Free Rasic Biol Med,1999,26(11/12):1531-1537.
• [6]SGAMBATO A,ARDITO R,FARAGLIA B,et al.Resveratrol,a naturalphenoliccompound,inhibits cell proliferation and prevents oxidative DNAdamage[J].Mutat Res,2001,496(12):171-180.
• [7]BURKITTMJ,DUNCANJ.Effects of trans-resveratrol on copper-depend-ent hydroxyl-radical formation and DNA damage:evidence for hydrocyl-radical scavenging and a novel,glutathione-sparing mechanism of action[J].Arch BiochemBiophy,2000,381(2):253-263.
• [8]任红涛,崔慧霞,赵秋燕.白藜芦醇提取工艺的优化与除杂方法的选择[J].食品工业科技,2005,26(7):99-101.
• [9]YUWL,SHU B,ZHAO Y P.Supercritical CO2extraction of resveratroland its glycoside piceid from Chinese traditional medicinal herbPolygo-num cuspidatum[J].J Science Food and Agriculture,2005,85(3):489-492.
• [10]李核,李攻科,张展霞.密闭式微波系统的微波辅助萃取动力学模型[J].中山大学学报,2004,43(3):40-44.