太子参药材总DNA提取研究Extraction of Genome DNA from Medicinal Materials of Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm.
王晓多;王晓玲;王晓丽;
摘要(Abstract):
[目的]从太子参干药材中快速提取高质量基因组DNA。[方法]采用CTAB法和SDS法2种方法提取药材太子参干燥块根总DNA,并用紫外分光光度计和琼脂糖凝胶电泳检测。[结果]2种不同的提取方法均可从太子参中提取到一定纯度的DNA,其中CTAB法提取DNA的A260/A280>1.9,纯度和产率较高,蛋白质、多糖、RNA等杂志去除较为彻底。[结论]利用CTAB法可从太子参干药材中能快速提取到高质量DNA。
关键词(KeyWords): 太子参;DNA;吸光度值
基金项目(Foundation):
作者(Author): 王晓多;王晓玲;王晓丽;
Email:
DOI: 10.13989/j.cnki.0517-6611.2012.29.272
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