应用于景观水体异养硝化细菌的筛选鉴定及效果研究Research on the Screening and Identification of Heterotrophic Nitrification Bacteria Applied in Landscape Water and Its Effect
苏俊峰;黃廷林;李倩;叶羡婧;魏全源;
摘要(Abstract):
[目的]研究景观水体的生物修复技术。[方法]采用SBR反应器,通过间歇曝气方式对底泥体系中好氧反硝化细菌进行了选择和富集,分离到1株异养硝化菌,并根据其生理生化性状和部分长度的16S rDNA序列确定了分离菌株的分类学和系统发育地位。[结果]从底泥中分离出1株异养硝化细菌SHW1,经过生理生化鉴定和16S rDNA测序,建立了系统发育树,鉴定出细菌SHW1属于Acineto-bactersp.。采用乙酸钠-氯化铵培养基培养细菌进行硝化特性研究,经过7 d好氧培养,NH4+-N最终去除率为52.13%,并且具有产生NO2--N的硝化性能。[结论]筛选出的异养硝化细菌SHW1在贫营养条件下对NH4+-N有较高的去除率,可以应用于景观水体脱氮。
关键词(KeyWords): 景观水体;异养硝化;硝化特性
基金项目(Foundation): 国家自然科学基金项目(50778147);; 教育部博士点新教师基金资助项目(20096120120013);; 陕西省自然科学基金资助项目(2010JQ7004);; 西安建筑科技大学青年科技基金资助项目(QN0916)
作者(Author): 苏俊峰;黃廷林;李倩;叶羡婧;魏全源;
Email:
DOI: 10.13989/j.cnki.0517-6611.2011.14.044
参考文献(References):
- [1]KENNISHM J.Environmental threats and environmental future of estuaries[J].Environ Conserv,2002,29:78-107.
- [2]王建华,潘伟斌.城区富营养化景观水体的生物修复技术[J].四川环境,2005,24(5):34-36.
- [3]KUENEN J G,ROBERTSON L A.Combined nitrification-denitrificationprocesses[J].FEMSM icrobiology Reviews,1994,15:109-117.
- [4]MURRAY I.Inter-relationships betwseen nitrogen balance,pH and dis-solved oxygen in an oxidation ditch treating farm animal waste[J].WatRes,1975,9:25-30.
- [5]何霞,吕剑,何义亮,等.异养硝化机理的研究进展[J].微生物学报,2006,46(5):844-847.
- [6]STEPHEN J R,KOWALCHUK G A,BRUN M V,et al.Analysis of a-sub-group proteobacteria ammoniaoxidizer populations in soil by denaturinggradient gel electrophoresis analysis and hierarchical phylogentic probing[J].Appl EnvironM icrobiol,1998,64:2958-2965.
- [7]林燕,孔海南,何义亮,等.异养硝化细菌的分离及其硝化特性实验研究[J].环境科学,2006,27(2):324-328.
- [8]ROBERTSON LA,NIEL EW J,TORREMANS R AM,et al.Simultaneousnitrification and denitrification in aerobic chemostat cultures ofThiospha-era pantotropha[J].Appl EnvM icrobiol,1988,54:2812-2818.
- [9]BRIERLEY M.Heterotrophic nitrif ication in an acid forest soil:isolationand characterisation of a nitrifying bacterium[J].Soil B iology&B iochem-istry,2001,33:1403-1409.
- [10]KSHIRSAGARM,GUPTA A B,GUPTA SK.Aerobic denitrification stud-ies on activated sludge m ixed withThiosphaera pantotropha[J].EnvironTech,1995,16:35-43.
- [11]苏俊峰,马放,王继华,等.新型异养硝化细菌的硝化和反硝化特性[J].天津大学学报,2007,40(10):1205-1208.