张益文;王连荣;张连成;张军;籍新波;王进茂;

• 1:河北农业大学林学院
• 2:河北北方学院园艺系
• 3:天津滨海新区塘沽林业工作站

摘要(Abstract)：

[目的]研究转基因抗虫棉Bt毒蛋白含量的时空变化及其土壤降解。[方法]采用ELISA法(酶链免疫法)研究和分析了转Bt-cry1Ac基因抗虫棉的根、茎和叶片组织在不同发育时期毒蛋白的含量变化及转Bt基因抗虫棉(GK45)和非转基因棉花(新陆早36号)在根际土壤、表层土壤和后茬种植区土壤中Btcry1Ac毒蛋白的年平均含量变化。[结果]BtCry1Ac毒蛋白含量在抗虫棉生长过程中均呈动态下降趋势,而根中下降的速率最快,茎和叶片次之;棉花种植区土壤表层中均检测到Btcry1Ac毒蛋白,且后茬种植区中表层毒蛋白的含量增加,而根际土中含量极低。[结论]Btcry1Ac毒蛋白的含量检测为种植转基因作物的风险评价及转基因作物的土壤生态系统安全性评价提供了科学依据。

关键词(KeyWords)： 转基因棉花;Bt毒蛋白;土壤微生物;安全性评价

Abstract:

Keywords:

基金项目(Foundation): 转基因生物新品种培育重大专项(2009ZX08011-027B);; 国家自然科学基金资助项目(30872050)

作者(Author): 张益文;王连荣;张连成;张军;籍新波;王进茂;

Email:

DOI: 10.13989/j.cnki.0517-6611.2012.25.096

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