长双歧杆菌NCC2705分泌蛋白的全基因组预测和功能分析Genome-wide and Function Analysis of the Secretory Proteins of Bifidobacterium longum NCC2705
朱德全;王茗悦;栗金柳;孟祥晨;程广东;岳丽红;王长平;薛勇;
摘要(Abstract)：
以长双歧杆菌NCC2705基因组序列为研究对象,使用Signal P 4.0、Lipo P、TMHMM 2.0软件分析该基因组中的分泌蛋白及其信号肽的类型,同时采用COG(Cluster of Orthologous Groups of proteins)功能数据库对预测的分泌蛋白进行功能注释和聚类分析。结果表明,长双岐杆菌NCC2705中共有37个Sec途径分泌蛋白,其中Sec途径分泌蛋白包括27个被I型(SPaseⅠ)信号肽酶和10个Ⅱ型信号肽酶(Spase II)识别的蛋白,Sec途径分泌蛋白信号肽长度最多的有52个氨基酸,最少的有10个氨基酸。分泌蛋白的功能分析表明,该菌株分泌蛋白中含有大部分的假定蛋白,主要参与氨基酸代谢与转运、碳水化合物代谢转运,无机盐离子代谢转运,细胞壁和细胞膜生物合成的功能有关。
关键词(KeyWords)： 长双歧杆菌;基因组;分泌蛋白;分泌途径;信号肽
基金项目(Foundation): 佳木斯大学科研项目(S2011-049);; 黑龙江省卫生计生委科研项目(2016-325)
作者(Author): 朱德全;王茗悦;栗金柳;孟祥晨;程广东;岳丽红;王长平;薛勇;
Email:
DOI: 10.13989/j.cnki.0517-6611.2016.20.035
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