7个微卫星标记对5个肉用绵羊品种的遗传多样性分析Genetic Diversity Analysis Among 3 Foreign Sheep Varieties and 2 Chinese Local Sheep Varieties by Using 7 Microsatellite Markers
汤晓良;瞿冬艳;陈仁金;毛永江;杨章平;
摘要(Abstract)：
[目的]为肉用绵羊的品种资源保护和杂交利用提供理论依据。[方法]利用7个微卫星标记分析国外3个绵羊品种和我国2个地方绵羊品种的遗传多样性,研究其品种内遗传变异和种间遗传关系。[结果]从5个绵羊品种中共检测到105个等位基因,在各群体中每个微卫星座位上均可检测到5个以上等位基因,说明7个微卫星标记含有丰富的遗传信息。位点平均杂合度和品种平均杂合度分别为0.790 1~0.889 7和0.795 1~0.867 5,属于高度杂合位点和高度杂合品种。7个位点均为高度多态位点,PIC为0.762 6~0.879 6,而5个绵羊品种的平均PIC为0.770 3~0.865 0。NJ系统树分析表明3个国外绵羊品种遗传关系较近。[结论]7个微卫星标记均具有高度多态性,可用于绵羊品种的遗传多样性和系统发生关系的分析。
关键词(KeyWords)： 绵羊;微卫星;遗传分化;系统地位
基金项目(Foundation):
作者(Author): 汤晓良;瞿冬艳;陈仁金;毛永江;杨章平;
Email:
DOI: 10.13989/j.cnki.0517-6611.2008.12.128
参考文献(References)：
- [1]TAUTZ D.Hypervariabilityof simplesequences as ageneral sourcefor polymor-phic DNAmarkers[J].Nuleic Acids Res,1989,17(16):6463-6467.
- [2]HAMADAH,PETRINOMG,KAKUNAGA,et al.Anovel repeatedelement withZ-DNA-forming potential is widely found in evolutionarily diverse eukaryoticgenomes[J].Proc Natl Sci USA,1982,79(21):6465-6469.
- [3]TAKEZAKI N,NEI M.Genetic distance andreconstructionof phylogenetictreesfrommicrosatellite DNA[J].Genetics,1996,144:389-399.
- [4]BOLSTEIND,WHITERL,SKOLICKM,et al.Constructionof a geneticlinkagemapin man usingrestrictionfragment length polymorphisms[J].Animal Jour-nal,1980,32:314-331.
- [5]杨燕,马月辉,吕慎金,等.中国7个地方绵羊品种遗传多样性的微卫星分析[J].生物多样性,2004,12(6):586-593.
- [6]贾斌,陈杰,赵茹茜,等.新疆8个绵羊品种遗传多样性和系统发生关系的微卫星分析[J].遗传学报,2003,30(9):847-854.
- [7]储明星,程金华,过纬.微卫星标记OarAE101和BM1329在五个绵羊品种中的初步研究[J].遗传学报,2001,28(6):510-517.
- [8]LUIKARTG,BIJUDUVAL MP,ERTUGRUL,et al.Power of 22 microsatellitemarkers influorescent multiplexesfor parentagetestingingoats[J].Animal Ge-netics,1999,30:431-438.
- [9]汪志国,杨章平,马月辉,等.长江中下游以及东南沿海的7个山羊群体的遗传多样性分析[J].畜牧兽医学报,2006,37(1):1-6.
- [10]储明星,王吉振,王爱国,等.小尾寒羊五个微卫星基因座遗传多态性研究[J].遗传学报,2002,29(6):502-506.
- [11]ARRANZ J J,BAYON Y,SANPRIMITIVO F.Genetic relationships amongSpanishsheep using microsatellites[J].Animal Genetics,1998,29(6):435-440.
- [12]ARRANZ J J,BAYONY,SANPRIMITIVOF.Differentiation among Spanishsheep breeds using microsatellite[J].Genetics Selection Evolution,2001,33(8):529-542.
- [13]STEPHENH,FORBESJ,HOGGT,et al.Microsatellite evolutionincongenericmammals:domestic and bighorn sheep[J].Molecular Biology and Evolution,1995,12:1106-1113.
- [14]SLATKIN M.Ameasure of populationsubdivision based on microsatellite allelefrequencies[J].Genetics,1995,139:457-462.