猪MSTN基因生物信息学分析Bioinformatics Analysis on MSTN Gene of Swine
王伟;连林生;李继中;
摘要(Abstract)：
[目的]对猪MSTN基因进行生物信息学分析。[方法]以从GenBank中检索到的猪、大鼠、小鼠、狗、绵羊、山羊、牛、黑猩猩、人、马、鸡和斑马鱼的MSTN基因CDS序列为材料,将该12个物种的MSTN蛋白序列调入DNAStar软件的Megalign程序,进行系统进化分析;并对猪MSTN基因的基本信息、内切酶图谱、编码蛋白二级结构、信号肽、跨膜结构以及蛋白质亚细胞定位进行分析。[结果]猪MSTN基因与大鼠、小鼠、狗、绵羊、山羊、牛、黑猩猩、人、马亲缘关系很近;该基因包含多个酶切位点;其编码的蛋白是一个疏水性不稳定蛋白,分子量为42 791.3 u,等电点为6.98,包含375个氨基酸残基;蛋白二级结构上,含有20.53%的α-螺旋(Helix)、4%β-转角(Turn)、53.07%无规则卷曲(Coil)、22.4%伸展条(extenden strand)和1个跨膜结构区域;该蛋白定位于细胞外,作为信号肽的可能性很大。[结论]该研究为猪MSTN基因的进一步分析研究提供了参考依据。
关键词(KeyWords)： 猪;MSTN;生物信息学
基金项目(Foundation): 国家自然科学基金项目(31060301)
作者(Author): 王伟;连林生;李继中;
Email:
DOI: 10.13989/j.cnki.0517-6611.2012.10.210
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