严帆;方平平;靳卫亚;

• 1:安徽师范大学花津校区

摘要(Abstract)：

[目的]探讨蔓越橘汁中植物性化学物质与生长环境中亚铁离子的螯合作用是否为蔓越橘汁抑菌的主要因素。[方法]采用革兰氏阳性菌金黄色葡萄球菌和革兰氏阴性菌大肠杆菌为对象,乙二胺四乙酸(EDTA)为参照物,利用平板涂布方法研究蔓越橘植物性化学物质与亚铁离子螯合作用对细菌生长的影响。[结果]随着亚铁离子溶液浓度的提高,蔓越橘汁固相萃取物组金黄色葡萄球菌和大肠杆菌的生长曲线相对低于EDTA组,甚至出现过量亚铁离子抑制细菌生长的现象。[结论]蔓越橘汁的抑菌原理主要来源于植物性多酚物质对细菌的作用,而非间接的与亚铁离子螯合作用。

关键词(KeyWords)： 蔓越橘;金黄色葡萄球菌;大肠杆菌;EDTA

Abstract:

Keywords:

基金项目(Foundation): 安徽师范大学青年教师科研专项(721355)

作者(Author): 严帆;方平平;靳卫亚;

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参考文献(References)：

• [1]杨静慧,李建科,杨恩芹,等.蔓越莓栽培历史、现状及发展趋势[J].天津农学院学报,2009,16(3):44-46.
• [2]衣晓峰.天然抗癌物——蔓越橘[J].抗癌之窗,2010(9):74.
• [3]王波,连奔,周琦,等.蔓越莓制剂抑制P菌毛阳性大肠埃希菌对红细胞黏附作用的研究[J].天津医药,2009,37(9):743-746.
• [4]SHMUELY H,OFEK I,WEISS E I,et al.Cranberry components for the therapy of infectious disease[J].Current opinion in biotechnology,2012,23(2):148-152.
• [5]PINZN-ARANGO P A,HOLGUIN K,CAMESANO T A.Impact of cranberry juice and proanthocyanidins on the ability of Escherichia coli to form biofilms[J].Food science and biotechnology,2011,20(5):1315-1321.
• [6]GUPTA A,DWIVEDI M,MAHDI A A,et al.Inhibition of adherence of multi-drug resistant E.coli by proanthocyanidin[J].Urological research,2012,40(2):143-150.
• [7]HOWELL K,YAN F,TOKICH A,et al.Iron sequestration is not the main mechanism in the inhibition of Staphylococcus aureus growth by cranberry phytochemicals[J].Integrative food,nutrition and metabolism,2015,2(3):184-188.
• [8]LIN B,JOHNSON B J,RUBIN R A,et al.Iron chelation by cranberry juice and its impact on Escherichia coli growth[J].Biofactors,2011,37(2):121
• [9]LACOMBE A,WU V C H,TYLER S,et al.Antimicrobial action of the American cranberry constituents;phenolics,anthocyanins and organic acids,against Escherichia coli O157:H7[J].International journal of food microbial,2010,139(1/2):102-107.
• [10]GUO M,PEREZ C,WEI Y,et al.Iron-binding properties of plant phenolics and cranberry’s bio-effects[J].Dalton transactions,2007,43(43):4951-4961.
• [11]CTJ,CAILLET S,DOYON G,et al.Bioactive compounds in cranberries and their biological properties[J].Critical reviews in food science and nutrition,2007,50:666-679.
• [12]CHUNG K T,LU Z,CHOU M W.Mechanism of inhibition of tannic acid and related compounds on the growth of intestinal bacteria[J].Food chemistry toxicology,1998,36(12):1053-1060.
• [13]WU V C H,QIU X,BUSHWAY A,et al.Antibacterial effects of American cranberry(Vaccinium macrocarpon)concentrate on foodborne pathogens[J].Food science and technology,2008,41(10):1834-1841.
• [14]PUUPPONEN-PIMIR,NOHYNEK L,MEIER C,et al.Antimicrobial properties of phenolic compounds from berries[J].Journal of applied microbiology,2001,90(4):494-507.