敖汉细毛羊Ⅰ型内根鞘角蛋白基因表达模式的研究分析Genechip Analysis of Type I IRS Keratin Expression Pattern in Aohan Wool Sheep
余娟娟;刘积凤;赵金山;程明;刘开东;柳楠;
摘要(Abstract)：
[目的]探讨细毛羊毛囊生长期及休止期特异基因的表达模式以及该模式对敖汉细毛羊选育的意义。[方法]通过基因芯片技术对敖汉细毛羊羊毛生长期和休止期的颈部、腹股沟部的上皮基因表达进行检测。[结果]统计分析Ⅰ型内根鞘角蛋白基因KRT25、KRT26、KRT27和KRT28在细毛羊颈部与腹股沟部的表达,发现该基因在细毛羊生长期颈部表达量极显著高于腹股沟部的表达量(差异倍数>2,P<0.01);对比分析生长期与休止期该基因的表达变化,结果显示在腹股沟部所有芯片涵盖的Ⅰ型内根鞘角蛋白基因的表达都表现出由生长期进入休止期的显著下调(P<0.05),除KRT25(LOC443079)外,其他基因的表达变化都在2倍以上。[结论]该研究结果表明Ⅰ型内根鞘角蛋白基因家族的表达与特异部位羊毛密度的控制以及整个毛囊发育生长周期都有着重要的联系。
关键词(KeyWords)： Ⅰ型内根鞘角蛋白;内根鞘;毛纤维;细毛羊;差异表达
基金项目(Foundation): 国家绒毛用羊产业技术体系(CARS-40-04)
作者(Author): 余娟娟;刘积凤;赵金山;程明;刘开东;柳楠;
Email:
DOI: 10.13989/j.cnki.0517-6611.2012.18.138
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