马铃薯X病毒外壳蛋白基因的克隆与系统进化分析Cloning and Phylogenetic Analysis of Potato Virus X CP Gene
黄永会;朱英;刘永翔;
摘要(Abstract)：
从贵州威宁等地采集马铃薯病叶样本,采用试管捕捉反转录PCR(tube-capture-RT-PCR,TC-RT-PCR)的方法克隆出714 bp的PVX CP基因,编码237个氨基酸残基。系统进化分析表明,PVX CP基因可分为两大类群,推测分离物属于X3株系,并建议重型花叶也可作为PVX病叶样本。
关键词(KeyWords)： 马铃薯X病毒;外壳蛋白;基因克隆;进化分析
基金项目(Foundation): 贵州省农业科学院研究生科研创新基金项目“抗病毒转基因马铃薯的荧光原位杂交(FISH)研究”[黔农科合(创新基金)2011011号];; 贵州省科研机构创新能力建设专项“药用植物和作物资源研究与开发应用创新能力建设”[黔科合院所创能[2010]4002]
作者(Author): 黄永会;朱英;刘永翔;
Email:
DOI: 10.13989/j.cnki.0517-6611.2014.27.012
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