球孢链霉菌JK-1抗菌物质的理化特性研究Study on Physicochemical Property of Antifungal Substances from Streptomyces globisporus JK-1
李其利;黄穗萍;郭堂勋;莫贱友;黄俊斌;
摘要(Abstract):
[目的]明确球孢链霉菌JK-1抗菌物质的基本理化性质。[方法]以稻瘟菌为指示菌,采用菌丝生长速率法分别测定球孢链霉菌JK-1发酵滤液在不同温度、不同酸碱度、蛋白酶K处理和硫酸铵盐析之后的抑菌活性;采用硫酸铵盐析对抗菌物质进行初步提纯,并通过超滤的方法初步测定该抗菌物质的分子量大小。[结果]球孢链霉菌JK-1产生的抗菌物质在高温和蛋白酶K处理的情况下抑菌活性显著降低,在pH2~11条件下抑菌活性无显著变化,而pH≥11的强碱性条件下抑菌活性显著降低。饱和度为50%的硫酸铵即可将发酵液中的抗菌物质完全沉淀下来,超滤发现其分子量在10~30 kD。[结论]该研究结果为球孢链霉菌的田间应用及其抗菌物质的分离鉴定奠定了基础。
关键词(KeyWords): 球孢链霉菌;抗菌物质;理化特性
基金项目(Foundation): 广西自然科学基金项目(2013GXNSFBA019075);; 广西农业科学院科技发展基金项目(桂农科2013JZ08)
作者(Author): 李其利;黄穗萍;郭堂勋;莫贱友;黄俊斌;
Email:
DOI: 10.13989/j.cnki.0517-6611.2014.27.042
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