猪圆环病毒多重PCR检测与分型Multiplex PCR Detection and Typing of Porcine Circovirus
易道生;娄高明;韩先干;冯顺胜;
摘要(Abstract)：
[目的]建立一种快速的PCV诊断方法,并能将2种PCV病毒区分开来,提供兽医临床和相关产品快速监测PCV的手段。[方法]根据genebank上公布的PCV1和PCV2序列,设计2对引物扩增PCV1和PCV2非同源性区域,建立快速检测PCV2个血清型的多重PCR,用已知的PCV1和PCV2及其他病毒细菌检测其敏感性和特异性。[结果]结果表明:所建立的方法特异、敏感,最低能检出0.02 ng的PCV1和PCV2混合DNA,用该法检测已知阳性病料和不同来源的猪血清,阳性符合率达100%。[结论]所建立的MultiplexPCR可用于PCV2个血清型的临床快速检测。
关键词(KeyWords)： 猪圆环病毒;多重PCR;检测;分型
基金项目(Foundation): 香港铭源基金会资助项目(314-140437)
作者(Author): 易道生;娄高明;韩先干;冯顺胜;
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DOI:
参考文献(References)：
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