邓勋;宋小双;宋瑞清;

• 1:东北林业大学林学院
• 2:黑龙江省森林保护研究所

摘要(Abstract)：

[目的]对姬松茸中分离的gpd启动子片段进行序列分析。[方法]根据报道的gpd启动子序列设计合成1对引物,以姬松茸基因组DNA为模板,采用PCR扩增法克隆得到了2条DNA特异性片段,并利用启动子信号扫描和启动子预测程序对2条序列的片段启动子区和转录结合位点进行分析。[结果]测序结果表明,2条序列分别为372 bp和614 bp,分别命名为J-gpd1和J-gpd2,GenBank登陆号分别为:EU220746和EU220747;J-gpd1有3个,J-gpd2有4个核心启动子区;2条序列不仅具有基本启动子作用元件CAATBOX和TATABOX外,还具有多个重要作用元件,如CGCGBOX、GATABOX、ASF-motif、W-BOX、TGACGT-motif和GTGA motif等。利用TFSEARCH ver.1.3分析表明,2条序列还含有多个转录因子结合位点,包括GCR1、ADR1、HSF、ABF1和RAP1等。[结论]研究推测,2条序列所启动的基因具有较高的多样性和准确性。

关键词(KeyWords)： 姬松茸;gpd启动子;克隆;序列分析

Abstract:

Keywords:

基金项目(Foundation): 黑龙江省自然科学基金资助项目(C200620)

作者(Author): 邓勋;宋小双;宋瑞清;

Email:

DOI: 10.13989/j.cnki.0517-6611.2009.03.098

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