林坤;周生波;王超;侍福梅;

• 1:聊城大学生命科学学院

摘要(Abstract)：

[目的]探讨微管结合蛋白MAP18在ABA调控拟南芥花粉生长中的作用,为进一步深入研究植物的极性发育模式提供线索。[方法]以拟南芥野生型(生态型Columbia,Col-0)及相关突变体花粉为试验材料,进行遗传学和药理学分析,研究花粉萌发和花粉管生长的调控因子。[结果]内源和外源ABA均显著抑制拟南芥花粉的萌发,但内源ABA对萌发的花粉管有促进伸长的作用。遗传学证据表明,微管结合蛋白MAP18过表达植株的花粉对ABA表现超敏感,而RNAi沉默株的花粉对ABA则表现出脱敏感现象,显示MAP18蛋白参与了ABA对花粉萌发及花粉管生长的调控过程。[结论]该试验结果为研究花粉萌发与花粉管生长调控模式提供了线索。

关键词(KeyWords)： 拟南芥;花粉;突变体;脱落酸;微管结合蛋白MAP18

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30800585)资助

作者(Author): 林坤;周生波;王超;侍福梅;

Email:

DOI: 10.13989/j.cnki.0517-6611.2012.13.004

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