吴冰洁;张永忠;吴玉厚;石雷;刘立科;郭尚敬;郭善利;姜闯道;

• 1:聊城大学生命科学学院
• 2:中国科学院植物研究所
• 3:烟台大学化学生物理工学院

摘要(Abstract)：

[目的]探讨红杨叶片发育过程中从幼叶到老叶的光合特性和花青素的关系。[方法]通过气体交换、叶绿素荧光和光谱技术研究了中华红杨叶片发育过程中的光合特性及与气孔发育的关系。[结果]①随着叶片的生长,花青素含量逐渐降低,叶绿素含量迅速增加。②光饱和光合速率随着叶片面积的增加而持续升高,但气孔导度的增加明显滞后。③增加CO2浓度时不同发育阶段的叶片净光合速率均有大幅度增加,但叶片发育后期光合速率增加程度较大。④光系统Ⅱ(PSⅡ)的最大光化学效率(Fv/Fm)在叶片发育过程中增加幅度较小(<15%)。[结论]叶片发育过程中存在明显的气孔限制;随着中华红杨叶片的生长光合机构逐步完善,叶绿素含量增加和花青素含量降低是叶片从红色逐渐变成绿色的主要原因。

关键词(KeyWords)： 中华红杨;光合作用;花青素;叶绿素荧光

Abstract:

Keywords:

基金项目(Foundation): 国家自然基金项目(30770223、30871455);; 北京市财政项目[京财预指(2008)第0178号]

作者(Author): 吴冰洁;张永忠;吴玉厚;石雷;刘立科;郭尚敬;郭善利;姜闯道;

Email:

DOI: 10.13989/j.cnki.0517-6611.2010.09.110

参考文献(References)：

• [1]姜闯道,高辉远,邹琦,等.田间大豆叶片成长过程中的光合特性及光破坏防御机制[J].植物生理与分子生物学学报,2004,30(4):428-434.
• [2]许大全.光合作用气孔限制分析中的一些问题[J].植物生理学通讯,1997,33(4):241-244.
• [3]GUENTHERJ E,MELISA.The physiological significance of photosystemⅡheterogeneity in chloroplasts[J].Photosynth Res,1990,23:105-109.
• [4]CHOINSKI J S,RALPHP,EAMUS D.Changes in photosynthesis during leaf expansion inCorymbia gummifera[J].AustJ Bot,2003,51:111-118.
• [5]JIANGCD,JIANGGM,WANGXZ,etal.Increased photosynthetic activ-ities and thermostability of photosystemⅡwith leaf development of elm seedlings(Ulmus pumila)probed by the fast fluorescence rise OJIP[J].Environmental and Experimental Botany,2006,58:261-268
• [6]DODD I C,CRITCHLEYC,WOODALL G S,et al.Photoinhibition in dif-ferently coloured juvenile leaves of Syzygiumspecies[J].Journal of Exper-imental Botany,1998,49,1437-1435.
• [7]CLOSEDC,BEADLECL,BROWNPH,etal.Cold-induced photoinhibi-tion affects establishment of Eucalyptusnitens(Deane&Maiden)Maiden angE.globulisLabill[J].Trees:Structure and Function,2000,15:32-41.
• [8]CLOSE D C,BEADLE C L.The Ecophysiology of foliar anthocyanin[J].The Botanical Review,2003,69(2):149-161.
• [9]HUGHES MH,MORLEYC B,SMITHWK.Coordination of anthocyanin decline and photosynthetic maturation in juvenile leaves ofthree deciduous tree species[J].NewPhytol,2007,175:675-685.
• [10]FARQUHAR GD,SHARKEY TD.Stomatal conductance and photosyn-thesis[J].Annu Rev Plant Physiol,1982,33:317-345.
• [11]GAMON J A,SURFUS J S.Assessing leaf pigment content and activity with a reflectometer[J].NewPhytol,1999,143:105-117.
• [12]姜闯道,高辉远,邹琦,等.叶角、光呼吸和热耗散协同作用减轻大豆幼叶光抑制[J].生态学报,2005,25(2):319-324.
• [13]LEBKUECHER J G,HALDEMANKA,HARRIS C E,et al.Development of photosystemⅡactivity during irradiance of etiolated Helianthus seed-lings[J].Amer J Bot,1999,86:1087-1092.
• [14]SRIVASTAVA A,STRASSER R J.Govindjee:Greening of peas:parallel measurements of77K emission spectra,OJIP chlorophyll a fluorescence transient,period four oscil-lation of the initial fluorescence level,delayed light emission,and P700[J].Photosyn Thetica,1999,37:365-392.
• [15]PIETRINI F,MASSACCI A.Leaf anthocyanin content Changes inZea maysL.Grown at low temperature:Significance for the relationship be-tween the quantumyield of PSⅡand the apparent quantumjield of CO2assimilation[J].Photosynt Res,1998,58:213-219.
• [16]STEYNWJ,WANDSJ E,HOLCROFTDM,etal.Anthocyanins in veg-etative tissues:a proposed unified function in photoprotection[J].New Phytologist,2002,155(3):349-361.