刘莹;韩锰;王文磊;马成玉;张沛;

• 1:河南省粮油饲料产品质量监督检验中心
• 2:华中农业大学作物遗传改良国家重点实验室
• 3:南京农业大学
• 4:洛阳师范学院生命科学学院

摘要(Abstract)：

高通量技术是以微型生物反应器为操作平台,实现对生物细胞的分离培养和分子测序。通过着重分析几种实用型高通量技术在微生物分离培养及分子测序中的应用进展,发现微包埋技术在高通量试验中的研究及应用较为深入;而对不同环境菌样的分离培养中,海洋微生物方面的应用最为突出。另外,通过对比高通量分子测序技术可以发现,Illumina测序法的平均测序速度最快;单分子测序法所得数据的精确度最高,测序读长最长,周期相对最短。最后,对高通量技术在微生物学应用中的一些不足进行总结,并提出假设性解决方法。

关键词(KeyWords)： 微生物;高通量技术;分离培养;分子测序

Abstract:

Keywords:

基金项目(Foundation): 国家自然基金青年项目(31701121);; 河南省科技公关项目(182102310667);; 河南省高等学校重点科研项目计划(18A180022)

作者(Author): 刘莹;韩锰;王文磊;马成玉;张沛;

Email:

DOI:

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