吕祝章;王文哲;梁青;邱丽娟;

• 1:日照职业技术学院
• 2:中国农业科学院作物研究所国家农作物基因资源与遗传改良重大科学工程实验室农业部作物种质资源与生物技术重点开放实验室

摘要(Abstract)：

[目的]鉴定大豆育成品种与其亲本间的遗传多样性差异和遗传多态性信息含量,为大豆育种提供参考。[方法]采用SSR标记技术对大豆育成品种与其亲本间的遗传多样性进行分析。[结果]野生大豆亲本总等位变异数和特有等位变异数分别是栽培大豆亲本的1.31倍和3.63倍;平均多态性信息含量由大到小依次为野生大豆亲本(0.545 0)、育成大豆品种(0.478 7)、栽培大豆亲本(0.415 6)。[结论]野生大豆的遗传背景复杂,遗传多样性丰富,其外部形态和内在遗传基础都与栽培大豆有明显差异。

关键词(KeyWords)： 野生大豆;育成品种;遗传多样性;等位变异;SSR

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 吕祝章;王文哲;梁青;邱丽娟;

Email:

DOI: 10.13989/j.cnki.0517-6611.2016.26.039

参考文献(References)：

• [1]张国栋.黑龙江省大豆品种系谱分析[J].大豆科学,1983,2(3):184-193.
• [2]盖钧镒,崔章林.中国大豆育成品种的亲本分析[J].南京农业大学学报,1994,17(3):19-23.
• [3]盖钧镒,赵团结,崔章林,等.中国1923-1995年育成的651个大豆品种的遗传基础[J].中国油料作物学报,1998,20(1):17-23.
• [4]张博,邱丽娟,常汝镇.中国大豆部分获奖品种与其祖先亲本间SSR标记的多态性比较和遗关系分析[J].农业生物技术学报,2003,11(4):351-358.
• [5]赵团结,崔章林,盖钧镒.中国大豆育成品种中江苏种质58-161的遗传贡献[J].大豆科学,1998,17(2):120-128.
• [6]POWELL W,MORGANTE M,ANDE C,et al.The comparison of RFLP,RAPD,AFLP and SSR(microsatellite)markers for germplasm analysis[J].Mol Breeding,1996,12:225-238.
• [7]POWELL W,MORGANTE M,DOYLE J J,et al.Genepool variation in genus glycine subgenus soja revealed by polymorphic nuclear and chloroplast microsatellites[J].Genetics,1996,144:793-803.
• [8]RUSSELL J R,FULLER J D,MACAULAU M,et al.Direct xomparison of levels of genetic variation among barley accessions detected by RFLPs,AFLPs,SSRs and RAPDs[J].Theor Apple Genet,1997,95:714-722.
• [9]MAUGHAN P J,SAGHAI MAROOF M A,BUSS G R.Microsatellite and amplified sequence length polymorphism in cultivated and wild soybean[J].Genome,1995,38:715-723.
• [10]MCGREGOR C E,LAMBERT C A,GREYLING M M,et al.A comparative assessment of DNA fingerprinting techniques(RAPD,ISSR,AFLP and SSR)in tetraploid potato(Solanum tuberosum L.)germplasm[J].Euphytica,2000,113(2):135-144.
• [11]RONGWEN J,AKKAYSA M S,BHAGWAT A A,et al.The use of microsatellite DNA markers for soybean genotype identification[J].Theor Appl Genet,1995,90:43-48.
• [12]DIWAN N,CREGAN P B.Automated sizing of fluorescent-labeled simple sequence repeat(SSR)markers to assay genetic variation in soybean[J].Theor Appl Genet,1997,95:723-733.
• [13]BROWN-GUEDIRA G L,THOMPSON J A,NELSON R L,et al.Evaluation of genetic diversity of soybean introductions and North american Ancestors using RAPD and SSR markers[J].Crop science,2000,40:815-823.
• [14]NARVEL J M,FEHR W R,CHU W C,et al.Simple sequence repeat diversity among soybean plant introductions and elite genotypes[J].Crop science,2000,40:1452-1458.
• [15]ABE J,XU D H,SUZUKI Y,et al.Soybean germplasm pools in Asia revealed by nuclear SSRs[J].Theor Appl Genet,2003,106:445-453.
• [16]关荣霞,常汝镇,邱丽娟.用于SSR分析的大豆DNA的快速提取[J].大豆科学,2003,21(1):73-74.
• [17]KEIM P,BEAVIS W,SCHUPP J,et al.Evaluation of soybean RFLP marker diversity in adapted germplasm[J].Theor Appl Genet,1992,85:205-212.
• [18]ZHANG X Y,LI L H,WANG H,et al.An estimation of the minimum number of SSR alleles needed to reveal genetic relationships in wheat varieties.I.Information from large-scale planted varieties and cornerstone breeding parents in Chinese wheat improvement and production[J].Thero Appl Genet,2002,106(1):112-117.
• [19]YOU G X,ZHANG X Y,WANG L F.An estimation of the minimum number of SSR loci needed to reveal genetic relationships in wheat varieties:Information from 96 random accessions with maximized genetic diversity[J].Mol Breeding,2004,14(4):397-406.
• [20]FRANCO J,CROSSA J,RIBAUT J M,et al.Method for combining molecular markers and phenotypic Attributes for classifying plant genotypes[J].Theor Appl Genet,2001,103:944-952.
• [21]SUN G L,DIAZ O,SALOMON B,et al.Genetic diversity in Elymus caninus as revealed by isozyme,RAPD,and microsatellite markers[J].Genome,1999,42(3):420-431.
• [22]DAVILA J A,LOARCE Y,RAMSAY L,et al.Comparison of RAMP and SSR markers for the study of wild barley genetic diversity[J].Hereditas,1999,131(1):5-13.
• [23]VIGOUROUS X Y,JAQUETH J S,MATSUOKA Y,et al.Rate and pattern of mutation at micro satellite loci in maize[J].Mol Bio Evol,2002,19:1251-1260.
• [24]LIU K,GOODMAN M,MUSE S,et al.Genetic structure and diversity among maize inbred lines as inferred from DNA microsatellites[J].Genetics,2003,165(4):2117-2128.