一种适宜于文库构建的土壤微生物总DNA提取方法A Suitable Total Microbial DNA Extraction Method for Building Genomic Library
高岳;
摘要(Abstract):
采用改良的方法提取2种土壤微生物总DNA,并采用透析法对提取的DNA进行纯化,与MO BIO公司Power Soil DNA isolation Kit试剂盒法进行比较,结果表明,改良的土壤微生物总DNA提取方法提取到的DNA明显高于试剂盒提取的DNA,且A260/A280及A260/A230与试剂盒提取的相差不大,片段也较大,是一种经济方便的土壤微生物总DNA提取方法,比较适合用于构建高质量的宏基因组文库。
关键词(KeyWords): 土壤微生物;透析法;DNA含量;宏基因组文库
基金项目(Foundation):
作者(Author): 高岳;
Email:
DOI: 10.13989/j.cnki.0517-6611.2014.34.012
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