高岳;

• 1:苏州农业职业技术学院

摘要(Abstract)：

采用改良的方法提取2种土壤微生物总DNA,并采用透析法对提取的DNA进行纯化,与MO BIO公司Power Soil DNA isolation Kit试剂盒法进行比较,结果表明,改良的土壤微生物总DNA提取方法提取到的DNA明显高于试剂盒提取的DNA,且A260/A280及A260/A230与试剂盒提取的相差不大,片段也较大,是一种经济方便的土壤微生物总DNA提取方法,比较适合用于构建高质量的宏基因组文库。

关键词(KeyWords)： 土壤微生物;透析法;DNA含量;宏基因组文库

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 高岳;

Email:

DOI: 10.13989/j.cnki.0517-6611.2014.34.012

参考文献(References)：

• [1]NEWMAN D J,CRAGG G M.Natural products as sources of new drugs over the last 25 years[J].J Nat Prod,2007,70:461-477.
• [2]HUGENHOLTZ P,GOEBEL B M,PACE N R.Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity[J].J Bacteriol,1998,180:4765-4774.
• [3]RAPPE M S,GIOVANNONI S J.The uncultured microbial majority[J].Annu Rev Microbiol,2003,57:369-394.
• [4]TORSVIK V,GOKSOYR J,DAAE F L.High diversity in DNA of soil bacteria[J].Appl Environ Microbiol,1990,56:782-787.
• [5]TORSVIK V,DAAE F L,SANDAA R A,et al.Novel techniques for analysing microbial diversity in natural and perturbed environments[J].J Biotechnol,1998,64:53-62.
• [6]BRADY S F,CLARDY J.Palmitoylputrescine,an antibiotic isolated from the heterologous expression of DNA extracted from bromeliad tank water[J].J Nat Prod,2004,67(8):1283-1286.
• [7]LIM H K,CHUNG E J,KIM J C,et al.Characterization of a forest soil metagenome clone that confers indirubin and indigo production on Escherichia coli[J].Appl Environ Microbiol,2005,71(12):7768-7777.
• [8]KIM J S,LIM H K,LEE M H,et al.Production of porphyrin intermediates in Escherichia coli carrying soil metagenomic genes[J].FEMS Microbiol Lett,2009,295(1):42-49.