一株H9N2亚型禽流感病毒全基因组序列的遗传变异分析Genetic Variation Analysis on the Whole Genomic Sequence of a H9N2 Subtype Avian Influenza Virus Isolate
于博;章振华;姜北宇;钱爱东;李林;景小冬;张建伟;
摘要(Abstract):
[目的]了解H9N2亚型禽流感病毒分离毒株A/Chicken/Hebei/WD/98(简称WD98)与其他参考毒株的遗传变异关系。[方法]采用RT-PCR技术扩增了WD98分离株8个基因片段的全基因序列,并将其与7个参考毒株进行同源性比较及遗传进化分析。[结果]分离株WD98与7个参考毒株全基因组同源性为91.1%~95.8%,与A/duck/Hong Kong/Y280/97的全基因组核苷酸同源性最高(95.8%),而与A/chicken/Pakistan/2/99同源性最低(91.1%)。WD98株的HA基因裂解位点为R-S-S-R↓G,其第226位受体结合位点为Q。[结论]禽流感病毒WD98毒株为低致病性禽流感病毒,不易感染人,与A/duck/HongKong/Y280/97亲缘关系最近,同属于欧亚种系A/Chicken/Beijing/1/94亚系。而在这同一亚系中,WD98株与A/Chicken/Beijing/1/94株的亲缘关系最远。
关键词(KeyWords): 禽流感病毒;H9N2;全基因组;遗传变异
基金项目(Foundation): 国家973资助项目子课题(2005CB523001)
作者(Author): 于博;章振华;姜北宇;钱爱东;李林;景小冬;张建伟;
Email:
DOI: 10.13989/j.cnki.0517-6611.2010.01.147
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