于博;章振华;姜北宇;钱爱东;李林;景小冬;张建伟;

• 1:吉林农业大学动物科技学院
• 2:北京市农林科学院畜牧兽医研究所

摘要(Abstract)：

[目的]了解H9N2亚型禽流感病毒分离毒株A/Chicken/Hebei/WD/98(简称WD98)与其他参考毒株的遗传变异关系。[方法]采用RT-PCR技术扩增了WD98分离株8个基因片段的全基因序列,并将其与7个参考毒株进行同源性比较及遗传进化分析。[结果]分离株WD98与7个参考毒株全基因组同源性为91.1%~95.8%,与A/duck/Hong Kong/Y280/97的全基因组核苷酸同源性最高(95.8%),而与A/chicken/Pakistan/2/99同源性最低(91.1%)。WD98株的HA基因裂解位点为R-S-S-R↓G,其第226位受体结合位点为Q。[结论]禽流感病毒WD98毒株为低致病性禽流感病毒,不易感染人,与A/duck/HongKong/Y280/97亲缘关系最近,同属于欧亚种系A/Chicken/Beijing/1/94亚系。而在这同一亚系中,WD98株与A/Chicken/Beijing/1/94株的亲缘关系最远。

关键词(KeyWords)： 禽流感病毒;H9N2;全基因组;遗传变异

Abstract:

Keywords:

基金项目(Foundation): 国家973资助项目子课题(2005CB523001)

作者(Author): 于博;章振华;姜北宇;钱爱东;李林;景小冬;张建伟;

Email:

DOI: 10.13989/j.cnki.0517-6611.2010.01.147

参考文献(References)：

• [1]唐秀英,付朝阳,冯菊艳.H9亚型禽流感的流行与防制[J].预防兽医学进展,2000,2(4):1-3.
• [2]GUO Y J,KRAUSS S,SENNE D A,et al.Characterization of the pathoge-nicity ofmembers of the newly established H9N2 influenza virus lineagesin Asia[J].Virology,2000,267:279-288.
• [3]甘孟侯.禽流感[M].北京:北京农业大学出版社,1995:13-80.
• [4]TASHIRO P,CIBOROWSKIH D,PULVERER K G,et al.Role of Staphy-lococcus protein in the development of influenza pneumonia[J].Nature,1987,325:536-537.
• [5]ROHM C,HORIMOTO T,KAWAOKA Y,et al.Do henagglutinin genes ofhighly pathogenic avain influen virus constitute unique phylogenetic linea-ges[J].Virology,1995,209:664-670.
• [6]KAWAOKA Y,WEBSTER R G.Sequence requirements for cleavage acti-vation of influenza virus hemagglutinin expressed in mammalian cells[J].Proc NatlAcad Sci,USA,1988,85:324-328.
• [7]WEISW,BRWN J H,CUSACK S,et al.Structure of the influenza virushamagglutinin complex with its receptor sialic acid[J].Nature,1988,333:426-431.
• [8]MATROSOVICH M N,KRAUSS S,WEBSTER R G.H9N2 influenza virusfrom poultry in Asia have human virus—like receptor specificity[J].Vi-rology,2001,281:156-162.
• [9]LIN Y P,SHAW M,GREGORY V,et al.Avain-to-human transm ission ofH9N2 subtype influenza viruses:Relationship between H9N2 and H5N1human isolates[J].Proc NatlAcad SciUSA,2000,97(17):9654-9658.
• [10]ROGERS G N,PAULSON J C,DANIELS R S,et al.Single am ino acidsubstitutions in influenza hae magglutinin change receptor binding speci-ficity[J].Nature,1983,304:76-78.