黄添毅;李亮;余雪芳;李欣欣;李永裕;吴少华;

• 1:福建农林大学园艺学院

摘要(Abstract)：

[目的]探讨脱落酸(Abscisic acid,简称ABA)分解途径关键酶基因(CYP707A)的特性,以期为揭示ABA调控梨花芽休眠进程的分子机制奠定基础。[方法]根据已公布的梨基因组数据库,通过本地blast的方法获得梨基因组CYP707A序列,运用DNAMAN软件设计引物,获得‘黄花梨’的2个脱落酸8’-羟化酶基因的CDS序列,分别命名为Pp CYP707A4(Genbank登录号:KP279630)和Pp CYP707A5(Genbank登录号:KP279631),并利用在线分析平台和生物软件进行生物信息学分析。[结果]Pp CYP707A4的CDS序列全长分别为1 431 bp,编码476个氨基酸;Pp CYP707A5的CDS序列全长为1 425 bp,编码474个氨基酸,两者同源性94.27%,氨基酸相似性94.96%,两者与苹果的2个基因在系统发育树上分别独自聚为一小支。[结论]Pp CYP707A4和Pp CYP707A5为同属于梨Pp CYP707A家族2个相似度较高的基因,它们可能发源于苹果和梨的共同祖先。

关键词(KeyWords)： 梨;休眠;CYP707A;克隆;序列分析

Abstract:

Keywords:

基金项目(Foundation): 高等学校博士学科点专项科研基金项目(201135151100-11);; 福建省科技计划重点项目(2012N0005);; 福建农林大学园艺学院青年学术骨干培养基金项目(FAFU2012-YYPY03)

作者(Author): 黄添毅;李亮;余雪芳;李欣欣;李永裕;吴少华;

Email:

DOI: 10.13989/j.cnki.0517-6611.2015.06.029

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