崔树军;张建云;谷立坤;和晶亮;武秀琴;张庆甫;

• 1:河南工程学院
• 2:中国科学院生态环境研究中心

摘要(Abstract)：

甲烷氧化菌以甲烷为其唯一的碳源和能源,在全球大气甲烷平衡中起着重要的作用,同时在污染治理方面具有潜在应用价值。总结了目前甲烷氧化菌的微生物分子生态学检测方法,分析了研究中取得的进展及存在的问题,并指出今后应加强研究的方面。

关键词(KeyWords)： 甲烷氧化;荧光原位杂交;磷脂脂肪酸;PCR-DGGE

Abstract:

Keywords:

基金项目(Foundation): 河南省科技攻关(092102310237)

作者(Author): 崔树军;张建云;谷立坤;和晶亮;武秀琴;张庆甫;

Email:

参考文献(References)：

• [1]BODELIERPLE,FRENZELP.Contribution ofmethanotrophic and nitrif-ying bacteria to CH4and NH4+oxidation in the rhizosphere of rice plantsas determined bynewmethods of discrimination[J].Applied and Environ-mental Microbiology,1999,65(5):1826-1833.
• [2]MACALADYJ L,MCMILLANAMS,DICKENSAF,etal.Population dy-namics of type I and II methanotrophic bacteria in rice soils[J].Environ-mental Microbiology,2002,4(3):148-157.
• [3]LIEBNER S,RUBLACK K,STUEHRMANN T,et al.Diversity of AerobicMethanotrophic Bacteria in a PermafrostActive LayerSoil ofthe Lena Del-ta,Siberia[J].Microbial Ecology,2009,57(1):25-35.
• [4]KOLB S,KNIEF C,DUNFIELD P F,et al.Abundance and activity of un-cultured methanotrophic bacteria involved in the consumption of atmos-pheric methane in two forest soils[J].Environmental Microbiology,2005,7(8):1150-1161.
• [5]SINGHBK,TATEKR,KOLIPAKAG,etal.Effectofafforestationand re-forestation of pastures on the activity and population dynamics of metha-notrophic bacteria[J].Applied and Environmental Microbiology,2007,73(16):5153-5161.
• [6]DINGH,VALENTINEDL.Methanotrophic bacteria occupybenthic micro-bial mats in shallowmarine hydrocarbon seeps,Coal Oil Point,California[J].Journal of Geophysical Research-Biogeosciences,2008,113(G1):1015-1021
• [7]HANSONRS,HANSONTE.Methanotrophic bacteria[J].MicrobiologicalReviews,1996,60(2):439-445.
• [8]MCDONALD I R,BODROSSY L,CHENY,et al.Molecular ecology tech-niques for the study of aerobic methanotrophs[J].Applied and Environ-mental Microbiology,2008,74(5):1305-1315.
• [9]闵航,谭玉龙,昊伟祥,等.一个厌氧甲烷氧化菌菌株的分离、纯化和特征研究[J].浙江大学学报:农业与生命科学版,2002,28(6):619-624.
• [10]王晓丽,于建国.一个甲烷氧化菌株的分离、鉴定及其特性研究[J].微生物学通报,2008,35(6):934-938.
• [11]TSUBOTAJ,ESHINIMAEVB T,KHMELENINAVN,et al.Methylother-mus thermalisgen.nov.,sp nov.,a novel moderatelythermophilic obligatemethanotroph froma hotspringin Japan[J].International Journal of Sys-tematic and Evolutionary Microbiology.2005,55:1877-1884.
• [12]SOROKIND Y,JONES B E,KUENENJ G.An obligate methylotrophic,methane-oxidizing Methylomicrobiumspecies froma highlyalkaline envi-ronment[J].Extremophiles,2000,4(3):145-155.
• [13]罗明芳,吴昊,王磊,等.甲烷氧化混合菌群MY9的生长特性[J].过程工程学报,2009,9(1):113-117.
• [14]TSUTSUMI M,KOJIMA H,UEMURA S,et al.Structure and activity ofsoil-inhabiting methanotrophic communities in northern forest of Japan[J].Soil Biology&Biochemistry,2009.41(2):403-408.
• [15]MCDONALD I R,KENNA E M,MURRELL J C.Detection of metha-nothophic bacteria in enviromental-samples with the PCR[J].Appliedand Environmental Microbiology,1995,61(1):116-121.
• [16]MIGUEZ CB,BOURQUED,SEALYJ A,etal.Detection and isolation ofmethanotrophic bacteria possessing soluble methane monooxygenase(sMMO)genes usingthe polymerase chain reaction(PCR)[J].Microbi-al Ecology,1997,33(1):21-31.
• [17]FUSE H,OHTA M,TAKIMURA O,et al.Oxidation of trichloroethyleneand dimethyl sulfide bya marine Methylomicrobiumstrain containingsol-uble methane monooxygenase[J].Bioscience Biotechnologyand Biochem-istry,1998,62(10):1925-1931.
• [18]AIT-BENICHOUS,JUGNIALB,GREER C W,et al.Methanotrophs andmethanotrophic activity in engineered landfill biocovers[J].Waste Man-agement,2009,29(9):2509-2517.
• [19]STEINKAMP R,ZIMMER W,PAPEN H.Improved method for detectionof methanotrophic bacteria in forestsoils byPCR[J].CurrentMicrobiolo-gy,2001,42(5):316-322.
• [20]TSIENHC,BRATINA B J,TSUJI K,et al.Use of oligodeoxynucleotidesignature probes for identification of physiological groups of methylotro-phic bacteria[J].Applied and Environmental Microbiology,1990,56(9):2858-2865.
• [21]HOLMES AJ,OWENS NJ P,MURRELLJ C.Detection of novel marinemethanotrophs using phylogenetic and functional gene probes after meth-ane enrichment[J].Microbiology,1995,141:1947-1955.
• [22]WISE MG,MCARTHUR J V,SHIMKETS LJ.Methanotroph diversity inlandfill soil:Isolation of novel type I and type II methanotrophs whosepresence was suggested by culture-independent 16S ribosomal DNAa-nalysis[J].Applied and Environmental Microbiology,1999,65(11):4887-4897.
• [23]AUMANAJ,STOLYAR S,COSTELLO AM,et al.Molecular character-ization of methanotrophic isolates from freshwater lake sediment[J].Ap-plied and Environmental Microbiology,2000,66(12):5259-5266.
• [24]GULLEDGEJ,AHMAD A,STEUDLERPA,et al.Family-and genus-lev-el 16S rRNA-targeted oligonucleotide probes for ecological studies ofmethanotrophic bacteria[J].Applied and Environmental Microbiology,2001,67(10):4726-4733.
• [25]DEDYSHS N,DUNFIELD P F,DERAKSHANI M,et al.Differential de-tection of type II methanotrophic bacteria in acidic peatlands usingnewlydeveloped 16S rRNA-targeted fluorescent oligonucleotide probes[J].Fems Microbiology Ecology,2003.43(3):299-308.