两歧双歧杆菌PRL2010菌体外表面蛋白的功能分析Functional Analysis of the Outer Surface Protein of Bifidobacterium bifidum PRL2010
朱德全;王茗悦;栗金柳;张跃华;张武;卢伟;栾积毅;刘向东;
摘要(Abstract):
[目的]分析两歧双歧杆菌PRL2010菌体外表面蛋白的功能。[方法]以两歧双歧杆菌PRL2010基因组序列为研究对象,采用Perry等人分析革兰氏阳性菌菌体外表面蛋白(PSE蛋白)的Inmembrane方法,同时采用COG功能数据库对预测的外表面蛋白进行功能注释和聚类分析。[结果]PRL2010外表面蛋白包括28个细胞壁蛋白、12个脂蛋白、182个细胞膜蛋白、38个分泌蛋白。PRL2010外表面蛋白的功能分析结果显示,大多数外表面蛋白与细胞壁、细胞膜的生物合成,无机离子转运与代谢,防御机制,碳水化合物转运与代谢,氨基酸转运与代谢等有关。[结论]该研究可为探讨该菌与宿主相互作用的分子机制奠定基础。
关键词(KeyWords): 两歧双歧杆菌;基因组;外表面蛋白;功能
基金项目(Foundation): 黑龙江省卫生计生委科研项目(2016-325);; 佳木斯大学科技创新团队项目(cxtd-2016-02)
作者(Author): 朱德全;王茗悦;栗金柳;张跃华;张武;卢伟;栾积毅;刘向东;
Email:
DOI: 10.13989/j.cnki.0517-6611.2017.18.037
参考文献(References):
- [1]SERAFINI F,STRATI F,RUAS-MADIEDO P,et al.Evaluation of adhesion properties and antibacterial activities of the infant gut commensal Bifidobacterium bifidum PRL2010[J].Anaerobe,2013,21:9-17.
- [2]ZANOTTI I,TURRONI F,PIEMONTESE A,et al.Evidence for cholesterollowering activity by Bifidobacterium bifidum PRL2010 through gut microbiota modulation[J].Appl Microbiol Biotechnol,2015,99(16):6813-6829.
- [3]TURRONI F,TAVERNITI V,RUAS-MADIEDO P,et al.Bifidobacterium bifidum PRL2010 modulates the host innate immune response[J].Applied and environmental microbiology,2014,80(2):730-740.
- [4]LIU C,ZHANG Z Y,DONG K,et al.Adhesion and immunomodulatory effects of Bifidobacterium lactis HN019 on intestinal epithelial cells INT-407[J].World journal of gastroenterology,2010,16(18):2283-2290.
- [5]GILAD O,SVENSSON B,VIBORG A H,et al.The extracellular proteome of Bifidobacterium animalis subsp.lactis BB-12 reveals proteins with putative roles in probiotic effects[J].Proteomics,2011,11(12):2503-2514.
- [6]TURRONI F,BOTTACINI F,FORONI E,et al.Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging[J].Proceedings of the national academy of sciences of the united states of america,2010,107(45):19514-19519.
- [7]赵文杰,曾嘉,柳建设,等.嗜酸氧化亚铁硫杆菌基因组分泌蛋白的初步分析[J].现代生物医学进展,2008,8(1):22-26.
- [8]周晓罡,侯思名,陈铎文,等.马铃薯晚疫病菌全基因组分泌蛋白的初步分析[J].遗传,2011,33(7):785-793.
- [9]周晓罡,李成云,赵之伟,等.粗糙脉孢菌基因组分泌蛋白的初步分析[J].遗传,2006,28(2):200-207.
- [10]BARINOV A,LOUX V,HAMMANI A,et al.Prediction of surface exposed proteins in Streptococcus pyogenes,with a potential application to other Gram-positive bacteria[J].Proteomics,2009,9(1):61-73.
- [11]PERRY A J,HO B K.Inmembrane,a bioinformatic workflow for annotation of bacterial cell-surface proteomes[J].Source code for biology and medicine,2013,8(1):9.
- [12]范成明,李成云,赵明富,等.根癌土壤杆菌C58 Cereon中分泌蛋白信号肽分析[J].微生物学报,2005,45(4):561-566.