两歧双歧杆菌PRL2010菌体外表面蛋白的功能分析Functional Analysis of the Outer Surface Protein of Bifidobacterium bifidum PRL2010
朱德全;王茗悦;栗金柳;张跃华;张武;卢伟;栾积毅;刘向东;
摘要(Abstract)：
[目的]分析两歧双歧杆菌PRL2010菌体外表面蛋白的功能。[方法]以两歧双歧杆菌PRL2010基因组序列为研究对象,采用Perry等人分析革兰氏阳性菌菌体外表面蛋白(PSE蛋白)的Inmembrane方法,同时采用COG功能数据库对预测的外表面蛋白进行功能注释和聚类分析。[结果]PRL2010外表面蛋白包括28个细胞壁蛋白、12个脂蛋白、182个细胞膜蛋白、38个分泌蛋白。PRL2010外表面蛋白的功能分析结果显示,大多数外表面蛋白与细胞壁、细胞膜的生物合成,无机离子转运与代谢,防御机制,碳水化合物转运与代谢,氨基酸转运与代谢等有关。[结论]该研究可为探讨该菌与宿主相互作用的分子机制奠定基础。
关键词(KeyWords)： 两歧双歧杆菌;基因组;外表面蛋白;功能
基金项目(Foundation): 黑龙江省卫生计生委科研项目(2016-325);; 佳木斯大学科技创新团队项目(cxtd-2016-02)
作者(Author): 朱德全;王茗悦;栗金柳;张跃华;张武;卢伟;栾积毅;刘向东;
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