大豆细菌性斑点病菌hrpZ_(Psg12)基因增强病原菌对大豆的致病性并引起烟草过敏性反应Enhancement of the Pathogenicity of Pathogen on Soybean and Cause of Tobacco Hypersensitive Response by hrpZ_(Psg12) Gene of Pseudomonas syringae pv. glycinea
张佳环;李娟;高洁;
摘要(Abstract):
[目的]明确hrpZPsg12基因对大豆细菌性斑点病菌致病性的影响。[方法]采用PCR方法从大豆细菌性斑点病菌中克隆hrpZPsg12基因,利用具有自杀特性的敲除质粒pKNOCK-Cm和具有功能互补作用的粘粒pUFR034,构建了hrpZPsg12基因的突变载体pKNOCK477-7和互补载体pUFR1026-68,并筛选出hrpZPsg12基因的突变体477-1及其功能互补子1026-5。进一步将野生型Psg12、突变体477-1和互补子反应斑。但是,反应斑的大小有差异,Psg12菌株接种的病斑较大,477-1的较小,互补子1026-5的接近野生型菌株Psg12。病斑中细菌繁殖量分析表明,野生型菌株Psg12繁殖量最高,突变体477-1的最低,互补子1026-5的接近野生型菌株Psg12的繁殖量。[结论]hrpZ-Psg12基因能够增强大豆细菌性斑点病菌对大豆的致病性,并且能够在烟草上产生过敏性反应。
关键词(KeyWords): 大豆细菌性斑点病菌;hrpZPsg12基因;突变体;致病性
基金项目(Foundation): 吉林农业大学科研启动基金资助项目“大豆细菌性斑点病菌HrpZPsgl2蛋白功能的研究”(384);; 农业部“十一五”转基因生物新品种培育重大专项“抗病虫转基因大豆新品种培育”(2008ZX08004-004)
作者(Author): 张佳环;李娟;高洁;
Email:
DOI: 10.13989/j.cnki.0517-6611.2011.15.174
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