赵军;

• 1:鲁东大学生命科学学院

摘要(Abstract)：

通过建立不同的培养条件,分别在含有外源性L-精氨酸(L-Arg)、一氧化氮合酶(NOS)抑制剂N-硝基-L精氨酸甲酯(N-nitro-L-ar-ginine methyl ester,L-NAME)以及外源性亚硝基铁氰化钠的培养液中对三角涡虫进行阶段培养,运用图像分析技术对三角涡虫的摄食行为进行动态追踪。经各项数据的统计与分析,认为NO对三角涡虫的摄食以及运动等行为都有一定程度的影响。

关键词(KeyWords)： 三角涡虫;涡虫行为;一氧化氮;一氧化氮合酶

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 赵军;

Email:

DOI: 10.13989/j.cnki.0517-6611.2012.09.006

参考文献(References)：

• [1]刘德增.中国淡水涡虫分类区系研究报告[J].国土与自然资源研究,1995(4):48-52.
• [2]刘昌利.淡水涡虫繁殖及再生的研究进展[J].皖西学院学报,2000,16(2):31-33.
• [3]李安萍.三角涡虫的生物学研究[J].太原师范专科学校学报,2001(3):23-25.
• [4]ANDERSEN J F,WEICHSEL A,BALFOUR C A,et al.The crystal struc-ture of nitrophorin 4 at 1.5 A resolution:transport of nitric oxide by a li-pocalin-based heme protein[J].Structure,1998,6(10):1315-1327.
• [5]AMANVERMEZ R,CELIK C.Effectiveness of free radicals in hydatid cysts[J].J Egypt Soc Parasitol,2002,32(1):259-269.
• [6]BROWNLEE D,HOLDEN-DYE L,WALKER R.The range and biologicalactivity of FMRFamide-related peptides and classical neurotransmitters innematodes[J].Adv Parasitol,2000,45:109-180.
• [7]STEFANO G B,OTTAVIANI E.The biochemical substrate of nitric oxidesignaling is present in primitive non-cognitive organisms[J].Brain Res,2002,924(1):82-89.
• [8]WHITTEN M,SUN F,TEW I,et al.Differential modulation of Rhodniusprolixus nitric oxide activities following challenge with Trypanosoma range-li,T.cruzi and bacterial cell wall components[J].Insect Biochem Mol Bi-ol,2007,37(5):440-452.
• [9]KATZOFF A,BEN-GEDALYA T,HURWITZ I,et al.Nitric oxide signalsthat aplysia have attempted to eat,a necessary component of memory for-mation after learning that food is inedible[J].J Neurophysiol,2006,96(3):1247-1257.
• [10]JACKLET J W,TIEMAN D G.Nitric oxide and histamine induce neuronalexcitability by blocking background currents in neuron MCC of Aplysia[J].J Neurophysiol,2004,91(2):656-665.
• [11]ELLIOTT C J,VEHOVSZKY A.Comparative pharmacology of feeding inmolluscs[J].Acta Biol Hung,2000,51(2/4):153-163.
• [12]ELPHICK M R,KEMENES G,STARAS K,et al.Behavioral role for nitricoxide in chemosensory activation of feeding in a mollusc[J].J Neurosci,1995,15(11):7653-7664.
• [13]COLASANTI M,LAURO G M,VENTURINI G.NO in hydra feeding re-sponse[J].Nature,1995,374(6522):505.
• [14]VOGT K S,GEDDES G C,BROSS L S,et al.Physiological characteriza-tion of stolon regression in a colonial hydroid[J].J Exp Biol,2008,211(Pt 5):731-740.
• [15]ERIKSSON K S.Nitric oxide synthase in the pharynx of the planarianDugesia tigrina[J].Cell Tissue Res,1996,286(3):407-410.
• [16]王晓安,蒋小满,刘新海.东亚三角头涡虫一氧化氮合酶的组织化学定位初步研究[J].四川动物,1999,18(2):51-52.
• [17]VIJAY S,RAWAT M,ADAK T,et al.Parasite killing in malaria non-vec-tor mosquito Anopheles culicifacies species B:implication of nitric oxidesynthase upregulation[J].PLoS One,2011,6(4):18400.
• [18]SERFOZO Z,ELEKES K.Nitric oxide level regulates the embryonic de-velopment of the pond snail Lymnaea stagnalis:pharmacological,behavior-al,and ultrastructural studies[J].Cell Tissue Res,2002,310(1):119-130.