响应面法优化重组毕赤酵母菌表达β-甘露聚糖酶的诱导条件Optimization of Expression Conditions of β-mannanase Induced by Recombinant Pichia pastoris by Response Surface Methodology
李慧玲;刘祖艳;赵敏;
摘要(Abstract)：
[目的]对重组毕赤酵母菌表达β-甘露聚糖酶的诱导条件进行优化。[方法]以重组毕赤酵母GS115为研究对象,通过单因素试验确定最佳产酶条件,并采用响应面试验确定3个因素的最佳水平。[结果]最佳产酶条件为:培养温度28℃,培养pH 6.5,摇床转速210 r/min,培养基装液量100 ml。响应面试验确定3个因素即装液量、甲醇添加量和pH最佳值分别为103.18 ml、1.77%和6.69;在此条件下,β-甘露聚糖酶的活力最大值为4 917.5 U/ml。[结论]验证试验证明模型预测值准确、可靠,为重组毕赤酵母菌的合理开发利用提供了依据。
关键词(KeyWords)： 重组毕赤酵母;β-甘露聚糖酶;响应面
基金项目(Foundation):
作者(Author): 李慧玲;刘祖艳;赵敏;
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