苋菜活性氧代谢对铅胁迫的响应Effects of Lead Stress on Active Oxygen Metabolism in Amaranth
杨敏文;柯世省;
摘要(Abstract):
[目的]了解铅对植物生长的抑制作用。[方法]以苋菜品种一点红为试材,采用盆栽试验,以醋酸铅配制土壤铅含量分别为0.1、0.2、0.4、0.8和1.6 mmol/kg干土,不加铅为对照,研究铅处理对苋菜生长、铅积累和活性氧代谢的影响。[结果]播种45 d后,铅胁迫(>0.4 mmol/kg)下苋菜的生长受到明显抑制,其中根受到的抑制程度最高。铅胁迫明显增加了苋菜器官中铅的含量,并主要积累在根部。随着铅处理浓度的增加,成熟叶超氧阴离子产生速率加快,过氧化氢含量、SOD和POD活性增加,CAT和APX活性降低,氧阴离子产生速率、过氧化氢含量、MDA含量和细胞电解质渗漏增加。[结论]铅胁迫引起苋菜活性氧代谢失衡,过氧化作用加剧,导致植物生长受阻。
关键词(KeyWords): 铅胁迫;生长分析;活性氧;抗氧化酶活性;苋菜
基金项目(Foundation): 浙江省自然科学基金资助项目(Y504256)
作者(Author): 杨敏文;柯世省;
Email:
DOI: 10.13989/j.cnki.0517-6611.2008.30.116
参考文献(References):
- [1]GOPAL R,RIZVI A H.Excess lead alters growth,metabolism and translocationof certain nutrients in radish[J].Chemosphere,2008,70:1539-1544.
- [2]UVEGES J L,CORBETT A L,MAL T K.Effects of Pb contamination on thegrowth ofLythrum salicaria[J].Environmental Pollution,2002,120:319-323.
- [3]QURESHI MI,ABDINMZ,QADIRS,et al.Lead-inducedoxidative stress andmetabolic alterationsinCassia angustifoliaVahl[J].Biologia Plantarum,2007,51:121-128.
- [4]ROMANOWSKAE,IGAMBERDIEVAU,PARYS E,et al.Stimulationof respi-rationby Pb2+in detachedleaves and mitochondria of C3and C4plants[J].Physiologia Plantarum,2002,116:148-154.
- [5]RULEYAT,SHARMANC,SAHI S V,et al.Effects of lead and chelators ongrowth,photosynthetic activityand Pb uptakeinSesbania drummondiigrowninsoil[J].Environmental Pollution,2006,144:11-18.
- [6]LIUD,LI TQ,YANGXE,et al.Effect of Pbonleaf antioxidant enzyme activi-ties and ultrastructure of thetwo ecotypes ofSedumalfrediiHance[J].RussianJournal of Plant Physiology,2008,55:68-76.
- [7]吴晓,熊治廷.不同品种苋菜对铅抗性的研究[J].武汉大学学报:理学版,2005,51(S2):294-296.
- [8]柯世省.铜对苋菜幼苗光合参数和活性氧代谢的影响[J].中国农业科学,2008,41(5):1317-1325.
- [9]黄昀,刘光德,李其林,等.农产品对土壤中重金属的富集能力研究[J].中国农学通报,2004,20(6):285-289.
- [10]杜应琼,何江华,陈俊坚,等.铅、镉和铬在叶类蔬菜中的累积及对其生长的影响[J].园艺学报,2003,30(1):51-55.
- [11]中国科学院上海植物生理研究所,上海市植物生理学会.现代植物生理学实验指南[M].北京:科学出版社,1999.
- [12]GIANNOPOLITIS C N,RIES S K.Superoxide dismutase II.Purification andquantitative relationship with water-soluble proteininseedlings[J].Plant Phys-iology,1977,59:315-318.
- [13]林植芳,李双顺,林桂珠,等.衰老叶片和叶绿体中H2O2的累积与膜脂过氧化的关系[J].植物生理学报,1988,14(1):16-22.
- [14]WUYX,VONTIEDEMANNA.Impact of fungicides onactive oxygenspeciesand antioxidant enzymesinspring barley(HordeumvulgareL.)exposedto o-zone[J].Environmental Pollution,2002,116:37-47.
- [15]T RKAN i,BORM,ZDEMIRF,et al.Differential responses oflipidperoxi-dation andantioxidantsintheleaves of drought-tolerantP.acutifoliusGrayanddrought-sensitiveP.vulgarisL.subjectedtopolyethyleneglycol mediated waterstress[J].Plant Science,2005,168:223-231.
- [16]MALKOWSKI E,KITA A,GALAS W,et al.Lead distributionincornseedlings(Zea maysL.)andits effect on growth andthe concentrations of potassiumand calcium[J].Plant Growth Regulation,2002,37:69-76.
- [17]KOPITTKEP M,ASHERCJ,KOPITTKE RA,et al.Toxic effects of Pb2+ongrowthof cowpea(Vigna unguiculata)[J].Environmental Pollution,2007,150:280-287.
- [18]SEREGINI V,IVANOVVB.Histochemical investigationof cadmiumandleaddistributionin plants[J].RussianJournal of Plant Physiology,1997,44:791-796.
- [19]TANHANP,KRUATRACHUE M,POKETHITIYOOKP,et al.Uptake and ac-cumulation of cadmium,lead and zinc by Siam weed[Chromolaena odorata(L.)King&Robinson][J].Chemosphere,2007,68:323-329.
- [20]SALINML.Toxic oxygenspecies andprotectivesystems ofthe chloroplast[J].Physiologia Plantarum,1988,72:681-689.
- [21]DEMIREVSKA-KEPOVA K,SIMOVA-STOILOVA L,STOYANOVA Z,et al.Biochemical changesinbarleyplants after excessivesupplyof copper and man-ganese[J].Environmental and Experimental Botany,2004,52:253-266.
- [22]DRAZKIEWICZ M,SKORZYNSKA-POLITE,KRUPAZ.Copper-induced ox-idative stress and antioxidant defence inArabidopsis thaliana[J].Biometals,2004,17:379-387.