潘朋歌;

• 1:保诺科技(北京)有限公司

摘要(Abstract)：

对NS1蛋白的进化、结构、调节病毒的致病性等进行了综述,在此基础上,对抗流感病毒药物及其防控技术进行了展望,以期为流感病毒的预防和治疗提供更多的可能性。

关键词(KeyWords)： A型流感病毒;非结构蛋白NS1;致病性;功能;结构

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 潘朋歌;

Email:

DOI:

参考文献(References)：

• [1]TREANOR J J,SNYDER M H,LONDON W T,et al.The B allele of the NS gene of avian influenza viruses,but not the A allele,attenuates a human influenza A virus for squirrel monkeys[J].Virology,1989,171(1):1-9.
• [2]LUDWIG S,SCHULTZ U,MANDLER J,et al.Phylogenetic relationship of the nonstructural(NS)genes of influenza A viruses[J].Virology,1991,183(2):566-577.
• [3]SUAREZ D L,PERDUE M L.Multiple alignment comparison of the nonstructural genes of influenza A viruses[J].Virus Res,1998,54(1):59-69.
• [4]WILLIAM G D,ADELAIDE M,GIOVANNI C,et al.Progressive truncation of the Non-structural 1 gene of H7N1 avian influenza viruses following extensive circulation in poultry[J].Virus Res,2006,119(2):171-176.
• [5]ENAMI M,ENAMI K.Characterization of influenza virus NS1 effector domain[J].Nat Struct Mol Biol,2000,74(12):5556-5561.
• [6]CHIEN C Y,TEJERO R,HUANG Y,et al.A novel RNA-binding motif in influenza A virus non-structural protein[J].Nat Sruct Biol,1997,4(11):891-895.
• [7]LIU J,LYNCH P A,CHIEN C Y,et al.Crystal structure of the unique RNA-binding domain of the influenza virus NS1 protein[J].Nat Struct Bio,1997,4(11):896-89.
• [8]FALCN A M,MARIN R M,ZRCHER T Z,et al.Defective RNA replication and late gene expression in temperature-sensitive Influenza viruses expressing deleted forms of the NS1 protein[J].Virol,2004,78(8):3880-3888.
• [9]郭莹莹,王靖飞.A型流感病毒NS1蛋白结构研究进展[J].生命科学,2010,22(2):116-118.
• [10]LIU J,LYNCH P A,CHIEN C Y,et al.Crystal structure of the unique RNA-binding domain of the influenza virus NS1 protein[J].Nat Struct Biol,1997,4(11):896-8999.
• [11]YIN C,KHAN J A,SWAPNA G V,et al.Conserved surface features form the double-stranded RNA binding site of non-structural protein 1(NS1)from influenza A and B viruses[J].Biol Chem,2007,282(28):20584-20592.
• [12]QIU Y,KRUG R M.The influenza virus NS1 protein is A poly(A)-binding protein that inhibits nuclear export of mRNAs containing poly(A)[J].Virol,1994,68(4):2425-2432.
• [13]LU Y,QIAN X Y,KRUG R M.The influenza virus NS1 protein:a novel inhibitor of pre-mRNA splicing[J].Genes Dev,1994,8(15):1817-1828.
• [14]QIU Y,NEMEROFF M,KRUG R M.The influenza virus NS1 protein binds to a specific region in human U6 snRNA and inhibits U6-U2 and U6-U4 snRNA interactions during splicing[J].RNA,1995,1(3):304-316.
• [15]BORNHOLDT Z A,PRASAD B V.X-ray structure of NS1 from a highly pathogenic H5N1 influenza virus[J].Nature,2008,456(7224):985-988.
• [16]XIA S,MONZINGO A F,ROBERTUS J D.Structure of NS1A effector domain from the influenza A/Udorn/72 virus[J].Acta Crystallogr D Biol Crystallogr,2009,65(1):11-17.
• [17]李建丽,张万坡,毕丁仁.A型流感病毒NS1蛋白研究进展[J].微生物学报,2007,47(4):729-733.
• [18]DAS K,MA L C,XIAO R,et al.Structural basis for suppression of a host antiviral response by influenza A virus[J].Proc Natl Acad Sci USA,2008,105(35):13093-13098.
• [19]LU Y M,WAMBACH M G,KATZE M,et al.Binding of the influenza virus NS1protein to double-stranded RNA inhibits the activation of the protein kinase that phosphorylates the e IF-2 translation initiation factor[J].Virology,1995,214:222.
• [20]TAN S L,KATZE M.Biochemical and genetic evidence for complex formation between the influenza A virus NS1 protein and the interferon-induced PKR protein kinase[J].J Interferon Cytokine Res,1998,18:757.
• [21]ABRAHAM N D F,STOJDL P I,DUNCAN N,et al.Characterization of transgenic mice with targeted disruption of the catalytic domain of the double-stranded RNA-dependent protein kinase,PKR[J].J Biol Chem,1999,274:5953.
• [22]NEMEROFF M E,BARABINO S M,LI Y,et al.Influenza virus NS1 protein interacts with the cellular 30 k Da subunit of CPSF and inhibits 3,end formation of cellular pre-mRNAs[J].Mol Cell,1988,1(7):991-1000.
• [23]CHEN Z,LI Y,KRUG R M.Influenza A virus NS1 protein targets poly(A)-binding proteinⅡof the cellular 39-end processing machinery[J].EMBO J,1999,18(8):2273-2283.
• [24]WOLFF T,O'NEILL R E,PALESE P.NS1-binding protein(NS1-BP):A novel human protein that interacts with the influenza A virus nonstructural NS1 protein is delocalized in the nuclei of infected cells[J].Journal of virology,1998,72(9):7170-7180.
• [25]ZHIRNOV O P,KONAKOVA T E,WOLFF T,et al.NS1 protein of influenza A virus down-regulates apoptosis[J].J Virol,2002,76(4):1617-1625.
• [26]EHRHARD T C,WOLFF T,PLESCHKA S,et al.The influenza A virus NS1 protein activates the PI3K/Akt pathway to mediate antiapoptotic signaling responses[J].J Virol,2007,81(7):3058-3067.
• [27]SMITH E J,MARI I,PRAKASH A,et al.IRF3 and IRF7 phosphorylation in virus-infected cells does not require double-stranded RNA-dependent protein kinase R or Ikapp B kinase but is blocked by vaccinia virus E3L protein[J].J Biol Chem,2001,276(12):8951-8957.
• [28]FERNANDEZ-SESMA A,SVETLANA M,BARBARA J E,et al.Influenza virus evades innate and adaptive immunity via the NS1 protein[J].J Virol,2006,80(13):6295-6304.
• [29]JULKUNEN I,SARENEVA T,PIRHONEN J,et al.Molecular pathogenesis of Influenza a virus infection and virus-induced regulation of cytokine gene expression[J].Cytokine Growth Factor Rev,2001,12:171.
• [30]FALCN A,FORTES P,MARIN R M.Interaction of influenza virus NS1protein and the human homologue of Staufen in vivo and in vitro[J].Nucleic Acids Res,1999,7(11):2241-2247.
• [31]WOLFF T,O'NEILL R E,PALESE P.Interaction cloning of NS1-I,a human protein that binds to the nonstructural NS1 proteins of influenza A and B viruses[J].J Virol,1996,70(8):5363-5372.