灰绿青霉阿魏酸酯酶的分离纯化和酶学性质研究Study on Separation,Purification and Enzymatic Properties of Ferulic Acid Esterase from Penicillium glaucum
徐大伟;高兆建;张铁柱;杨杰;王东星;杜永凯;
摘要(Abstract):
[目的]研究青霉(Penicillium glaucum)XGY36胞外产阿魏酸酯酶的纯化和酶学性质。[方法]菌株胞外产酶发酵液经硫酸铵沉淀、DEAE-cellulose DE52离子交换柱层析、Sephadex-G75分子筛层析进行纯化,得到纯度较高的酶蛋白,并对其酶学性质进行研究。[结果]从菌株发酵液中分离纯化到电泳纯的阿魏酸酯酶,纯化倍数6.54,酶活性回收59.7%。阿魏酸酯酶经SDS-PAGE获得1个条带,其表观分子量为36.5 ku。催化底物阿魏酸乙酯的最适反应温度为50℃,最适p H为5.0。阿魏酸酯酶在60℃以下和p H 3.0~7.0范围内稳定。金属离子Zn~(2+)、Mg~(2+)、Ca~(2+)对酶有明显的激活作用,而Pb~(2+)、Hg~(2+)和Ag+对阿魏酸酯酶有强烈的抑制作用,Na+、K+、Mn~(2+)、Cu~(2+)和Fe~(2+)对酶活的影响不大。[结论]青霉阿魏酸酯酶具有潜在的应用价值,适合应用于食品、医药及生物等领域。
关键词(KeyWords): 灰绿青霉;阿魏酸酯酶;纯化;酶学性质
基金项目(Foundation): 大学生创新创业基金项目(201311998053Y);; 徐州市科技计划项目(XF13C011,XF13C027)
作者(Author): 徐大伟;高兆建;张铁柱;杨杰;王东星;杜永凯;
Email:
DOI: 10.13989/j.cnki.0517-6611.2016.15.008
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