王青柏;李良秋;徐鹏;曾绮文;王东东;王嘉铭;谭雨婷;

• 1:广东省微生物研究所广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室省部共建华南应用微生物国家重点实验室

摘要(Abstract)：

创伤弧菌(Vibrio vulnificus)是一种重要的水产品污染病原,可造成养殖水产品的病害,同时也是一种致命的人类机会致病菌,与全世界大部分食用污染海鲜致死的案例有关。随着全球气温升高,以及食用海鲜产品的人群变多,我国创伤弧菌感染率呈现上升趋势。简要综述了创伤弧菌的生物学特性及分子检测的最新进展,为创伤弧菌感染的早期诊断和及时治疗提供参考资料。

关键词(KeyWords)： 创伤弧菌;生物学特性;检测技术

Abstract:

Keywords:

基金项目(Foundation): 广东省省级科技计划项目(2013B06180004)

作者(Author): 王青柏;李良秋;徐鹏;曾绮文;王东东;王嘉铭;谭雨婷;

Email:

DOI:

参考文献(References)：

• [1] BROSS M H,SOCH K,MORALES R,et al.Vibrio vulnificus infection:Diagnosis and treatment[J].Am Fam Physician,2007,76(4):539-544.
• [2] STROM M S,PARANJPYE R N.Epidemiology and pathogenesis of Vibrio vulnijkus Microbes [J].Infect,2000,2(2):177-188.
• [3] 姜红.原发性创伤弧菌败血症一例[J].中华内科杂志,1991(30):645-646,127.
• [4] CHUNG P H,CHUANG S K,TSANG T,et al.Cutaneous injury and Vibrio vulnificus infection[J].Emerg Infect Dis,2006,12(8):1302-1303.
• [5] 林燕.1例创伤弧菌前臂感染病人的护理[J].全科护理,2018(7):890-892.
• [6] 肖美英,沈芳,王莺,等.1例创伤弧菌创面感染后继发感染性休克的分析[J].中国卫生检验杂志,2017(11):1571-1573.
• [7] 邓慧倍.8例创伤弧菌感染患者的护理[J].护理学报,2017(8):33-34.
• [8] 郑小庆,盛吉芳.创伤弧菌感染13例临床特点及预后分析[J].中国微生态学杂志,2016,28(2):213-217.
• [9] 陈晓燕,胡超群.溶藻弧菌脂多糖在两种鱼类体内的组织分布[J].热带海洋学报,2002,21(4):30-35.
• [10] CHEN C Y,CHAO C B,BOWSER P R.Infection of tilapia Oreochromis sp.by Vibrio vulnificus in freshwater and low-salinity environments[J].World Aquacult Soc,2006,37(1):82-88.
• [11] FOUZ B,AMARO C.Isolation of a new serovar of Vibrio vulnificus pathogenic for eels cultured in freshwater farms [J].Aquaculture,2003,217(1/2/34):667-682.
• [12] LI G F,ZHAO D H,HUANG L,et al.Identification and phylogenetic analysis of Vibrio vulnificus isolated from diseased Trachinotus ovatus in cage mariculture[J].Aquaculture,2006,261(1):17-25.
• [13] SI L N,LI S W,WANG D.The isolation and drug sensitive tests of pathogens found in sturgeon epizootic disease[J].Chin J Fish,2010,23(4):18-22.
• [14] ABDULLAH A,RAMLI R,RIDZUAN M S M,et al.The presence of Vibrionaceae,Betanodavirus and Iridovirus in marine cagecultured fish:Role of fish size,water physicochemical parameters and relationships among the pathogens[J].Aquac Rep,2017,7:57-65.
• [15] LIU R R,LIAN Z Y,HU X C,et al.First report of Vibrio vulnificus infection in grass carp Ctenopharyngodon idellus in China[J].Aquaculture,2019,499:283-289.
• [16] TAMPLIN M,RODRICK G E,BLAKE N J,et al.Isolation and characterization of Vibrio vulnificus from two Florida estuaries[J].Appl Environ Microbial,1982,44(6):1466-1470.
• [17] HENG S P,LETCHUMANAN V,DENG C Y,et al.Vibrio vulnificus:An environmental and clinical burden[J].Front Microbial,2017,8:1-25.
• [18] HORI M,NAKAYAMA A,KITAGAWA D,et al.A case of Vibrio vulnificus infection complicated with fulminant purpura:Gene and biotype analysis of the pathogen[J].JMM Case Reports,2017,4(5):1-3.
• [19] KIM H R,RHO H W,JEONG M H,et al.Hemolytic mechanism of cytolysin produced from V.vulnificus[J]. Life Sci,1993,53(7):571-577.
• [20] YAMANAKA H,SATOH T,KATSU T,et al.Mechanism of haemolysis by Vibrio vulnificus haemolysin[J].Journal of general microbiology,1987,133(10):2859-2864.
• [21] KASHIMOTO T,UENO S,EHARA H,et al.Oligomerization is essential for apoptotic activity of Vibrio vulnificus hemolysin[J].Vet Med Sci,2009,71(10):1403-1406.
• [22] GULIG P A,BOURDAGE K L,STARKS A M.Molecular pathogenesis of Vibrio vulnificus[J].J Microbiol,2005,43:118-131.
• [23] LALLY E T,HILL R B,KIEBA I R,et al.The interaction between RTX toxins and target cells[J].Trends microbiol,1999,7(9):356-361.
• [24] LO H R,LIN J H,CHEN Y H,et al.RTX toxin enhances the survival of Vibrio vulnificus during infection by protecting the organism from phagocytosis[J].J Infect Dis,2011,203(12):1866-1874.
• [25] JEONG H G,SATCHELL K J F.Additive function of Vibrio vulnificus MARTX(Vv)and VvhA cytolysins promotes rapid growth and epithelial tissue necrosis during intestinal infection[J].PLoS Pathog,2012,8(3):1-13.
• [26] LITWIN C M,RAYBACK T W,SKINNER J.Role of catechol siderophore synthesis in Vibrio vulnificus virulence[J].Infect Immun,1996,64(7):2834-2838.
• [27] SIMPSON L M,OLIVER J D.Siderophore production by Vibrio vulnificus[J].Infect Immun,1983,41(2):644-649.
• [28] KIM I H,SHIM J I,LEE K E,et al.Nonribosomal peptide synthase is responsible for the biosynthesis of siderophore in Vibrio vulnificus MO6-24/O[J].J Microbiol Biotechnol,2008,18(1):35-42.
• [29] CHATZIDAKI-LIVANIS M,JONES M K,WRIGHT A C.Genetic variation in the Vibrio vulnificus group lcapsular polysaccharide operon [J].J Bacterio,2006,188(5):1987-1998.
• [30] HILTON T,ROSCHE T,FROELICH B,et al. Capsular polysaccharide phase variation in Vibrio vulnificus[J]. Appl environ microbiol,2006,72(11):6986-6993.
• [31] 徐宸,王鑫,钟江.创伤弧菌快速检测法的建立[J].安徽农业科学,2018,46(19):114-116.
• [32] HILL W E,KEASLER S P,TRUCKSESS M W,et al.Polymerase chain reaction identification of Vibrio vulnificus in artificially contaminated oysters [J].Appl Environ Microbiol,1991,57(3):707-711.
• [33] KUMAR H S,PARVATHI A,KARUNASAGAR I,et al.A gyrB-based PCR for the detection of Vibrio vulnificus and its application for direct detection of this pathogen in oysterenrichment broths[J].Int J Food Microbiol,2006,111(3):216-220.
• [34] 张新中,张世秀,李海平,等.多重PCR在创伤弧菌快速检测中的应用[J].水产科学,2007,26(12):668-670.
• [35] LEE C Y,PANICKER G,BEJ A K.Detection of pathogenic bacteria in shellfish using multiplex PCR followed by CovaLink NH microwell plate sandwich hybridization[J].J Microbiol Methods,2003,53(2):199-209.
• [36] BAUER A,R?RVIK L M.A novel multiplex PCR for the identification of Vibrio parahaemolyticus,Vibrio cholerae and Vibrio vulnificus[J].Lett Appl Microbiol,2007,45(4):371-375.
• [37] TARR C L,PATEL J S,PUHR N D,et al.Identification of Vibrio isolates by a multiplex PCR assay and rpoB sequence determination[J].J Clin Microbiol,2007,45(1):134-140.
• [38] 苏琰,李融,黎华.基于双启动寡聚核苷酸引物的PCR技术及其研究应用[J].基因组学与应用生物学,2018,37(7):2906-2912.
• [39] CHUN J Y,KIM K J,HWANG I T,et al.Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and SNP genotyping of CYP2C19 gene[J].Nucleic acids research,2007,35(6):1-6.
• [40] XU Y G,SUN L M,WANG Y S,et al.Simultaneous detection of Vibrio cholerae,Vibrio alginolyticus,Vibrio parahaemolyticus and Vibrio vulnificus in seafood using dual priming oligonucleotide(DPO)system-based multiplex PCR assay [J].Food control,2017,71:64-70.
• [41] ARYA M,SHERGILL I S,WILLIAMSON M,et al.Basic principles of real-time quantitative PCR[J].Expert review of molecular diagnostics,2005,5(2):209-219.
• [42] 王紫薇,汪琦,赵晓娟,等.2016年北京市海产品中创伤弧菌的污染调查及两种检测方法比较[J].中国食品卫生杂志,2018,30(2):182-186.
• [43] 刘军.食源性致病菌定量检测技术研究近况[J].中国卫生检验杂志,2016,26(2):302-304.
• [44] 国家食品安全风险评估中心.2015年国家食品污染和有害因素风险监测工作手册[M].北京:中国标准出版社,2015:684-877.
• [45] BONNY S Q,HOSSAIN M A M,LIN T K,et al.Multiplex MPN-PCR for the enumeration of three major Vibriosin raw fishes in Malaysia [J].Food control,2018,90:459-465.
• [46] NOTOMI T,OKAYAMA H,MASUBUCHI H,et al.Loop-mediated isothermal amplification of DNA[J].Nucleic Acids Res,2000,28(12):E63.
• [47] REN C H,HU C Q,LUO P,et al.Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification[J].Microbiol Res,2009,164(5):514-521.
• [48] 别闯南,王权,白雪瑞.检测创伤弧菌两种毒力基因的环介导等温扩增检测方法的建立[J].中国动物传染病学报,2018,26(1):48-54.
• [49] 刘彤,郑秋月,赵昕,等.变性高效液相色谱技术对创伤弧菌检测的研究[J].中国微生态学杂志,2011,23(5):470-472.
• [50] STROM M S,PARANJPYE R N.Epidemiology and pathogenesis of Vibrio vulnificus[J].Microbes Infect,2000,2(2):177-188.
• [51] HARWOOD V J,GANDHI J P,WRIGHT A C.Methods for isolation and confirmation of Vibrio vulnificus from oysters and environmental sources:A review[J].J Microbiol Methods,2004,59(3):301-316.