铅诱导蚕豆根尖细胞微核及染色体畸变的研究Effects of Pb~(2+) on Micronucleusn and Chromosomal Aberration of Vicia faba
张英慧;
摘要(Abstract):
[目的]研究铅对蚕豆根尖细胞微核及染色体畸变的影响。[方法]用不同浓度的Pb Cl2溶液培养蚕豆种子,以蒸馏水培养作为对照,培养4~7 d时,每天随机取根尖固定、染色、压片后镜检,观察微核和染色体畸变,计算微核率和染色体畸变率。[结果]培养第4~7天,1.0×10-4~5.0×10-4mol/L Pb2+处理组的微核率和染色体畸变率均极显著高于对照组,且Pb2+浓度越大,微核率和染色体畸变率越高。[结论]Pb2+对蚕豆根尖细胞微核率和染色体畸变率有影响,其影响程度与Pb2+处理浓度和处理时间有关。
关键词(KeyWords): 铅;蚕豆;微核;染色体畸变
基金项目(Foundation): 西藏自治区科技厅自然科学基金项目(12KJZRYMY08)
作者(Author): 张英慧;
Email:
DOI: 10.13989/j.cnki.0517-6611.2017.27.002
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