秦艳杰;马俊峰;李霞;张慧敏;

• 1:大连海洋大学水产与生命学院

摘要(Abstract)：

抑制性消减杂交技术(SSH)是近年来兴起的一种分离、克隆差异基因的新技术。系统阐述了该技术的原理和优缺点,综述了该技术在水产无脊椎动物差异表达基因筛选方面应用的研究进展,并对该领域面临的问题进行剖析。

关键词(KeyWords)： 抑制性消减杂交技术;水产无脊椎动物;研究进展

Abstract:

Keywords:

基金项目(Foundation): 辽宁省教育厅重点实验室项目(2008S064);辽宁省教育厅创新团队项目(2007T015)

作者(Author): 秦艳杰;马俊峰;李霞;张慧敏;

Email:

DOI: 10.13989/j.cnki.0517-6611.2012.09.221

参考文献(References)：

• [1]WAN J,MATTHEW B W,LI C,et al.Efficacy of SSH PCR in isolating dif-ferentially expressed genes[J].B M C Genomics,2002,3(12):1-7.
• [2]REBRIKOV D V,BRITANOVA O V,GURSKAYA N G,et al.Mirror orien-tation selection(MOS):a method for eliminating false positive clones fromlibraries generated by suppression subtractive hybridization[J].NucleicAcids Research,2000,28(20):91-94.
• [3]王兵,桂朗,李富花,等.抑制性消减杂交技术结合cDNA MICROAR-RAY技术在中国明对虾WSSV感染后差异表达基因研究上的应用[J].海洋与湖沼,2008,39(5):455-461.
• [4]WANG B,LI F,DONG B,et al.Discovery of the genes in response to whitespot syndrome virus(WSSV)infection in Fenneropenaeus chinensis throughcDNA microarray[J].Mar Biotechnol,2006,8(5):491-500.
• [5]HE N,QIN Q,XU X.Differential profile of genes expressed in hemocytes ofwhite spot syndrome virus-resistant shrimp(Penaeus japonicus)by combi-ning suppression subtractive hybridization and differential hybridization[J].Antiviral Res,2005,66(1):39-45.
• [6]ZHAO Z Y,YIN Z X,WENG S P,et al.Profiling of differentially expressedgenes in hepatopancreas of white spot syndrome virus-resistant shrimp(Litopenaeus vannamei)by suppression subtractive hybrzation[J].FishShellfish Immunolt,2007,22(5):520-534.
• [7]JAMES R,THAMPURAN N,LALITHA K V,et al.Differential gene ex-pression profile of the hepatopancreas of white spot syndrome virus infectedFenneropenaeus indicus by suppression subtractive hybridization[J].Fish&Shellfish Immunology,2010,29(5):884-889.
• [8]黎铭,陈晓汉,熊建华,等.感染与未感染桃拉病毒凡納滨对虾基因差异表达文库的构建[J].西南农业学报,2010,23(1):234-238.
• [9]LORGERIL J D,SAULNIER D,JANECH M G,et al.Identification of genesthat are differentially expressed in hemocytes of the pacific blue shrimp(Litopenaeus stylirostris)surviving an infection with Vibrio penaeicida[J].Physiol Genomics,2005,21(2):174-183.
• [10]GROSS P S,BARTLETT T C,BROWDY C L,et al.Immunegene discover-y by expressed sequence tag analysis of hemocytes and hepatopancreas inthe pacific white shrimp,Litopenaeus vannamei,and the atlantic whiteshrimp,L.setiferus[J].Dev Comp Immunol,2001,25(7):565-577.
• [11]NAYAK S,AJAY K M,RAMAIAH N,et al.Profiling of a few immune re-sponsive genes expressed in postlarvae of Fenneropenaeus indicus chal-lenged with Vibrio harveyi D3[J].Journal of Invertebrate Pathology,2011,107:168-172.
• [12]NAYAK S,SINGH S K,RAMAIAH N,et al.Identification of upregulatedimmune-related genes in Vibrio harveyi challenged Penaeus monodon post-larvae[J].Fish&Shellfish Immunology,2010,29:544-549.
• [13]HE N,LIU H,XU X.Identification of genes involved in the response ofhaemocytes of Penaeus Japonicus by suppression subtractive hybridization(SSH)following microbial challenge[J].Fish Shellfish Immunol,2004,17(2):121-128.
• [14]RENAULT T,FAURY N,BARBOSA-SOLOMIEU V,et al.Suppressionsubstractive hybridisation(SSH)and real time PCR reveal differentialgene expression in the Pacific cupped oyster,Crassostrea gigas,challengedwith Ostreid herpesvirus 1[J].Dev Comp Immunol,2011,5:725-735.
• [15]GREEN TJ,DIXON TJ,DEVIC E,et al.Differential expression of genesencoding anti-oxidant enzymes in Sydney rock oysters,Saccostrea glomera-ta(Gould)selected for disease resistance[J].Fish&Shellfish Immunolo-gy,2009,26:799-810.
• [16]TRAVERS M A,MEISTERTZHEIM A L,CARDINAUD M,et al.Gene ex-pression patterns of abalone,Halotis tuberculata,during successive infec-tions by the pathogen Vibrio harveyi[J].Journal of Invertebrate Patholo-gy,2010,105:289-297.
• [17]ZHANG Y,FU D,YU F,et al.Two homologues of catalase genes are in-volved in host protection against bacterial infection and oxidative stress inCrassostrea hongkongensis[J].Fish Shellfish Immunol,2011,31:894-903.
• [18]GESTAL C,COSTA M,FIGUERAS A.Analysis of differentially expressedgenes in response to bacterial stimulation in hemocytes of the carpet-shellclam Ruditapes decussatus:Identification of new antimicrobial peptides[J].Gene,2007,406:134-143.
• [19]ARAYA M T,MARKHAM F,MATEO D R,et al.Identification and ex-pression of immune-related genes in hemocytes of soft-shell clams,Myaarenaria,challenged with Vibrio splendidus[J].Fish&Shellfish Immunol-ogy,2010,29(4):557-564.
• [20]WANG K J,REN H L,XU D D,et al.Identification of the up-regulatedexpression genes in hemocytes of variously colored abalone(Haliotis diver-sicolor Reeve,1846)challenged with bacteria[J].Developmental andComparative Immunology,2008,32:1326-1347.
• [21]MARTN-GMEZ L,VILLALBA A,ABOLLO E.Identification and ex-pression of immune genes in the flat oyster Ostrea edulis in response tobonamiosis[J].Gene,2012,492(1):81-93.
• [22]TANGUY A,GUO X,FORD S E.Discovery of genes expressed in re-sponse to Perkinsus marinus challenge in Eastern(Crassostrea virginica)and Pacific(C.gigas)oysters[J].Gene,2004,338(1):121-131.
• [23]TANGUY A,PRADO-ALVAREZ M,GESTAL C.Differentially expressedgenes of the carpet shell clam Ruditapes decussates against Perkinsus olseni[J].Fish&Immunology,2009,26:72-83.
• [24]KANG Y S,KIM Y M,PARK K I.Analysis of EST and lectin expressionsin hemocytes of Manila clams(Ruditapes philippinarum)(Bivalvia:Mol-lusca)infected with Perkinsus olseni[J].Dev Comp Immunol,2006,30:1119-1131.
• [25]GRIFFITT R J,CHANDLER G T,GREIG T W,et al.Cathepsin B andglutathione peroxidase show differing transcriptional responses in the grassshrimp,Palaemonetes pugio following exposure to three xenobiotics[J].Environ Sci Technol,2006,40(11):3640-3645.
• [26]DE LA VEGA E,DEGNAN B M,HALL M R,et al.Differential expressionof immune-related genes and transposable elements in black tiger shrimp(Penaeus monodon)exposed to a range of environmental stressors[J].Fish Shellfish Immunol,2007,23(5):1072-1088.
• [27]HUVET A,HERPIN A,DEGREMONT L,et al.The identification of genesfrom the oyster Crassostrea gigas that are differentially expressed in proge-ny exhibiting opposed susceptibility to summer mortality[J].Gene,2004,343:211-220.
• [28]DAVID E.TANGUY A,PICHAVANT K,et al.Response of the Pacificoyster Crassostrea gigas to hypoxia exposure under experimental condi-tions[J].FEBS J,2005,272:5635–5652.
• [29]FU D K,CHEN J H,ZHANG Y,et al.Cloning and expression of a heatshock protein(HSP)90 gene in the haemocytes of Crassostrea hongkon-gensis under osmotic stress and bacterial challenge[J].Fish&ShellfishImmunology,2011,31:118-125.
• [30]MEDEIROS I D,SIEBERT M N,TOLEDO-SILV A G,et al.Differentialgene expression in oyster exposed to sewage[J].Marine EnvironmentalResearch,2008,66(1):156-157.
• [31]SILVA-ACIARES F,ZAPATA M,TOURNOIS J,et al.Identification ofgenes expressed in juvenile haliotis rufescens in response to different cop-per concentrations in the north of Chile under controlled conditions[J].Marine Pollution Bulletin,2011,62:2671-2680.
• [32]ZAPATA M,TANGUY A,DAVID E.Transcriptomic response of Argopect-en purpuratus post-larvae to copper exposure under experimental condi-tions[J].Gene,2009,442:37-46.
• [33]BROWN M,DAVIES I M,MOFFAT C F,et al.Application of SSH and amacroarray to investigate altered gene expression in Mytilus edulis in re-sponse to exposure to benzo[a]pyrene[J].Mar Environ Res,2006,62:128-135.
• [34]刘晶,张文兵,麦康森,等.皱纹盘鲍外套膜耐维生素E缺乏消减cD-NA文库的构建[J].中国水产科学,2007,14(3):383-389.
• [35]王嘉,马洪明,麦康森,等.皱纹盘鲍肝胰腺和肾脏维生素C缺乏诱导的差异表达cDNA文库的构建[J].中国水产科学,2010,17(4):619-629.
• [36]XU W,FAISAL M.Development of a cDNA microarray of zebra mussel(Dreissena polymorpha)foot and its use in understanding the early stageof underwater adhesion[J].Gene,2009,436:71-80.
• [37]ZHANG Z,WANG Y,JIANG Y,et al.Ribosomal protein l24 is differenti-ally expressed in ovary and testis of the marine shrimp Marsupenaeus ja-ponicus[J].Bioehem Mol Biol,2007,147(3):466-474.
• [38]XIE Y S,LI F H,WANG B.Screening of genes related to ovary develop-ment in Chinese shrimp Fenneropenaeus chinensis by suppression subtrac-tive hybridization[J].Comparative Biochemistry and Physiology,Part D,2010,5:98-104.
• [39]TANGPRASITTIPAP A,TIENSUWAN M,WITHYACHUMNARNKUL B.Characterization of candidate genes involved in growth of black tigershrimp Penaeus monodon[J].Aquaculture,2010,307:150-156.