产气荚膜梭菌α-β融合基因的构建与表达研究Study on the Construction and Expression of the α-β Fusion Gene
马同锁;李翠莲;赵士豪;张红兵;赵宝华;
摘要(Abstract)：
应用PCR技术,首先从A型产气荚膜梭菌菌株NCTC64609中扩增出α-毒素保护性抗原基因片段,然后从C型产气荚膜梭菌C58-1染色体基因组中扩增了930 bp的β-毒素基因,并将α-毒素基因和β-毒素基因插入融合表达载体pBV221中,获得了重组质粒pMCPAB,转化受体菌BL21(DE3),得到重组菌株BL21(DE3)(pMCPAB)。对重组菌株诱导的表达产物进行ELISA检测和SDS-PAGE分析,结果表明重组菌株可以高效表达α-β融合蛋白。免疫实验表明,表达产物免疫的小鼠可抵抗α-毒素和β-毒素的攻击。
关键词(KeyWords)： 产气荚膜梭菌;α-毒素;β-毒素;融合基因;基因表达
基金项目(Foundation):
作者(Author): 马同锁;李翠莲;赵士豪;张红兵;赵宝华;
Email:
DOI: 10.13989/j.cnki.0517-6611.2006.19.016
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