茯苓硫酸酯化多糖的制备及其核磁共振波谱分析Preparation and NMR Spectral Characterization of Pachyman Sulfate
陈群;
摘要(Abstract):
[目的]用不同方法制备3种茯苓多糖硫酸酯(Pachyman sulfate,PS),并对其进行核磁共振波谱对比分析,为多糖硫酸化提供基础数据。[方法]以氯磺酸为磺化试剂硫酸化茯苓多糖制备茯苓多糖硫酸酯PSⅠ-和PSⅡ-,以氨基磺酸为磺化试剂制备PSⅢ-,分析磺化反应前后和不同硫酸根取代度(Degree of sulfation,DS)的多糖碳原子化学位移的变化规律。[结果]PSⅠ-(DS=2.80)分子所有自由羟基均发生取代反应;PⅡS-(DS=1.55)C-6羟基被全部酯化,C-2和C-4羟基发生部分酯化反应;PSⅢ-(DS=0.95)C-6和C-2羟基发生部分硫酸酯化反应。[结论]发生取代反应的碳原子化学位移移动的距离与PS的DS呈正相关,核磁共振技术能够提供多糖结构解析的关键数据。
关键词(KeyWords): 茯苓多糖;硫酸化;茯苓多糖硫酸酯;核磁共振波谱分析
基金项目(Foundation): 合肥学院引进人才启动基金资助项目(600821)
作者(Author): 陈群;
Email:
DOI: 10.13989/j.cnki.0517-6611.2010.01.157
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