里氏木霉纤维素酶水解稻草的研究Enzymatic Hydrolysis of Rice Straw by Trichoderma reesei Cellulase
顾斌涛;黄朝;黄国昌;邱小忠;吕珺;张婷;
摘要(Abstract)：
[目的]探讨碳氮源对里氏木霉发酵产纤维素酶的影响,以及纤维素酶水解稻草的条件。[方法]通过添加不同的碳源和不同浓度的酵母粉,探讨里氏木霉合适的发酵条件;使用不同添加量的纤维素酶对稻草进行酶解;分别利用纤维素酶、纤维素酶和木聚糖酶混合酶对稻草进行酶解反应。[结果]利用乳糖和稻草的复合碳源和12 g/L的酵母粉进行水解时,纤维素酶活性较高。酶解适宜的酶用量为每克稻草底物200 U的滤纸酶。用纤维素酶及木聚糖酶混合酶酶解稻草96 h的酶解得率为65.4%。[结论]该研究可为里氏木霉纤维素酶生产和酶解稻草的应用提供一定的依据。
关键词(KeyWords)： 纤维素酶;里氏木霉;稻草酶解
基金项目(Foundation): 江西省科研院所基础设施配套项目(20142BBA13030);; 江西省科学院科研开发专项(2014-YYB-01);江西省科学院协同创新专项资金普惠一类项目(2013-XTPH-29)
作者(Author): 顾斌涛;黄朝;黄国昌;邱小忠;吕珺;张婷;
Email:
DOI:
参考文献(References)：
- [1]LIU K M,DONG Y M,WANG F Z,et al.Regulation of cellulase expression,sporulation,and morphogenesis by velvet family proteins in Trichoderma reesei[J].Appl Microbiol Biotechnol,2016,100(2):769-779.
- [2]JUTURU V,WU J C.Microbial cellulases:Engineering,production and applications[J].Renew and Sust Energ Rev,2014,33(6):188-203.
- [3]DASHTBAN M,MAKI M,LEUNG K T,et al.Cellulase activities in biomass conversion:Measurement methods and comparison[J].Crit Rev Biotechnol,2010,30(4):302-309.
- [4]何敏超,许敬亮,袁振宏,等.纤维素酶基因表达研究进展[J].林产化学与工业,2014,34(5):169-174.
- [5]ATREYA M E,STROBEL K L,CLARK D S.Alleviating product inhibition in cellulase enzyme Cel7A[J].Biotechnol Bioeng,2016,113(2):330-338.
- [6]方诩,秦玉琪,李雪芝,等.纤维素酶与木质纤维素生物降解转化的研究进展[J].生物工程学报,2010,26(7):864-869.
- [7]LI J B,ZHOU P F,LIU H M,et al.Ethanol production from xylan-removed sugarcane bagasse using low loading of commercial cellulase[J].Bioresource technology,2014,163(7):390-394.
- [8]SINGHANIA R R,PATEL A K,SUKUMARAN R K,et al.Role and significance of beta-glucosidases in the hydrolysis of cellulose for bioethanol production[J].Bioresource technology,2013,127(1):500-507.
- [9]HU J G,ARANTES V,SADDLER J N.The enhancement of enzymatic hydrolysis of lignocellulosic substrates by the addition of accessory enzymes such as xylanase:is it an additive or synergistic effect?[J].Biotechnology for biofuels,2011,4(1):36.
- [10]SCHUSTER A,SCHMOLL M.Biology and biotechnology of Trichoderma[J].Appl Microbiol Biotechnol,2010,87(3):787-799.
- [11]黄晓梅,杨谦,范金霞,等.绿色木霉内切葡聚糖酶基因I的克隆表达[J].哈尔滨工业大学学报,2010,42(12):1921-1926.
- [12]FOREMAN P K,BROWN D,DANKMEYER L,et al.Transcriptional regulation of biomass-degrading enzymes in the filamentous fungus Trichoderma reesei[J].The journal of biological chemistry,2003,278(34):31988-31997.
- [13]张鑫,刘岩.木质纤维原料预处理技术的研究进展[J].纤维素科学与技术,2005,13(2):54-58.
- [14]黄涛,程康华,王传槐.吐温-80及碱预处理对饲用稻草木质素含量和纤维素酶解产糖的影响[J].纤维素科学与技术,2011,19(2):52-58.