槲皮素对BEL-7402细胞生长抑制作用的研究Study on the Anti-proliferation Effect of Quercetin on the Growth of BEL-7402 Cells
马朋;
摘要(Abstract)：
[目的]探讨槲皮素对人肝癌细胞株BEL-7402的增殖抑制及凋亡诱导作用。[方法]体外培养BEL-7402细胞,以不同浓度槲皮素处理细胞后,采用酸性磷酸酶(APA)法检测细胞增殖抑制率;光镜观测BEL-7402细胞形态变化;流式细胞仪检测细胞凋亡率。[结果]槲皮素对人肝癌BEL-7402细胞有明显的增殖抑制作用,其72h的IC50为4μg/ml。光学显微镜下可见细胞密度明显降低。经槲皮素作用6、12h后,流式细胞术检测到细胞凋亡率显著增加。[结论]槲皮素对人肝癌细胞的增殖抑制呈浓度相关性,可诱导肝癌细胞凋亡。
关键词(KeyWords)： 槲皮素;肝癌;细胞凋亡
基金项目(Foundation):
作者(Author): 马朋;
Email:
DOI: 10.13989/j.cnki.0517-6611.2010.11.166
参考文献(References)：
- [1]李云峰,郭长江.槲皮素代谢的研究进展[J].生理科学进展,2002,33(1):53-55.
- [2]NGUYENTT,TRANE,NGUYENTH,et al.The role of activated MEK-ERK pathway in quercetin induced growth inhibition and apoptosis in A549lung cancer cells[J].Carcinogenesis,2004,25:647-659.
- [3]WENZEL U,HERZOG A,KUNTZ S,et al.Protein expression profiling i-dentifies molecular targets of quercetin as a major dietary flavonoid in hu-man colon cancer cells[J].Proteomics,2004,4:2160-2174.
- [4]刘明学,吴凯南,赵小波.槲皮素联合奥曲肽对人乳腺癌细胞株MDA-MB-231增生的影响[J].中国肿瘤临床,2005,32(7):404-406.
- [5]苑辉卿,杨玲玲,姜安丽,等.槲皮素对前列腺癌细胞增殖及转录因子Spl功能的抑制作用[J].中国生物化学与分子生物学报,2005,21(5):673-678.
- [6]KUOHM,HOHJ,CHAOP D,et al.Quercetin glucuronides inhibited2-aminofluorene acetylation in human acute myeloid HL-60leukemia cells[J].Phytomedicine,2002,9:625-631.
- [7]陈学清,杨晓鸥,潘国宗,等.酸性磷酸酶法在体外化疗药物筛选中的应用[J].癌症,1998,17(6):477-478.
- [8]陈罡,王晓冬.细胞凋亡检测技术的进展[J].国外医学:分子生物学分册,2003,25(2):125-128.
- [9]WOO K,MYUNG H,EUL S,et al.Quercetin decreases the expressionof ErbB2and ErbB3proteins in HT-29human colon cancer ceI1s[J].J Nutr Biochem,2005,16(6):155-162.
- [10]MERTENS-TALCOTTS U,PERCIVAL S S.Ellagic acid and quercetin interact synergistically with resveratrol in the induction of apoptosis and cause transient cell cycle arrest in human leukenlia cells[J].Cancer Lett,2005,218(12):141-151.
- [11]MICHITAKAN,YUT,HIROYUKI N,et al.Quercetin,a flavonoid,inhib-its the proliferation,differentiation,and mineralization of osteoblasts in vitro[J].Eur J Phammcol,2004,485(9):89-96.
- [12]DECHSUPAS,KOTHANS,VERGOTE J,et al.Quercetin,siamois1and siamois2induce apoptosis in human breast cancer MDA-MB-435cells xenograft in vivo[J].Cancer Biol Ther,2007,6(1):56-61.
- [13]CONKLINC M,BECHBERGER J F,MACFABE D,et al.Genistein and quercetin increase connexin43and suppress growth of breast cancer cells[J].Carcinogenesis,2007,28(1):93-100.