双向电泳过程中的常见问题及解决方法Common Problems and Resolutions among 2-Dimensional Electrophoresis
舒海燕;曹刚强;凌华;袁保梅;田保明;
摘要(Abstract)：
[目的]研究蛋白质组双向电泳中出现的问题及解决方法。[方法]培养野生型K12菌株Escherichia coli MG1655,分离其蛋白质样品并进行SDS-PAGE聚丙烯酰胺凝胶电泳。[结果]双向电泳中存在蛋白质点没有出现或很少,纵纹与横纹现象严重等问题。[结论]该研究为以后开展双向电泳试验打下了基础。
关键词(KeyWords)： 双向电泳;影响因素;解决方法;蛋白质组
基金项目(Foundation):
作者(Author): 舒海燕;曹刚强;凌华;袁保梅;田保明;
Email:
DOI: 10.13989/j.cnki.0517-6611.2009.03.021
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