橡胶草种植前后土壤微生物细菌多样性研究(Ⅱ)——高级分析Research of Soil Microbial Bacteria Diversity before and after Planting Taraxacum kok-saghyz(Ⅱ)——Advanced Analysis
梁素钰;李琳;杜倩;刘铁男;王文帆;田松岩;
摘要(Abstract):
[目的]研究橡胶草种植前后土壤微生物细菌的多样性。[方法]对大田种植橡胶草前后的土壤理化性质进行考察,并通过454测序技术对土壤微生物细菌多样的数据序列进行高级分析。[结果]橡胶草种植后土壤的全氮和全磷略高于种植前,有机质稍有下降。土壤细菌的OTU数在橡胶草种植前后相差不大。群落结构分析表明,橡胶草种植前后的细菌组成大致相同,但各物种所占比例有差异,大多数的细菌是不可培养的,其中与氮有关的菌属柱状区所占比例最大。PCA主成分分析表明橡胶草种植前后土壤微生物群落在细菌水平上相近,这个结果与群落分布柱状图相吻合。RDA分析表明,土壤pH值、有机质、土壤含水率、土壤全氮和全磷与细菌呈正相关,土壤容重与细菌呈负相关。[结论]试验测序数据表明,橡胶草种植前后的土壤微生物细菌在OUT水平上多样性丰富,在属的水平上群落结构组成相近,种植橡胶草后土壤理化性质发生了改变。
关键词(KeyWords): 橡胶草(Taraxacum kok-saghyz Rodin);454测序;细菌;多样性;土壤微生物
基金项目(Foundation): 国家林业局948,编号2011-4-51;; 黑龙江省财政,编号2011-02;; 黑龙江省森工总局,编号sgzjY2010020;; 黑龙江省博士后,编号LBH-Q10010
作者(Author): 梁素钰;李琳;杜倩;刘铁男;王文帆;田松岩;
Email:
DOI: 10.13989/j.cnki.0517-6611.2014.08.001
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