家鸡胚胎发育过程DNA甲基化的MSAP检测Detection of MSAP(Methylation Sensitive Amplified Polymorphism) on DNA Methylation during Chicken Fetation
班谦;赵宗胜;曹体婷;
摘要(Abstract)：
[目的]检测鸡胚不同发育阶段时的DNA甲基化水平。[方法]以不同发育阶段的家鸡胚胎为试验材料,提取基因组DNA,采用甲基敏感扩增片段多态性(methylation sensitive amplified polymorphism,MSAP)技术对其甲基化水平进行了初步检测。[结果]共发现甲基化位点880个,CCGG位点单链外侧的胞嘧啶甲基化位点比例为20.48%,双链CCGG位点的内侧胞嘧啶甲基化19.30%,两者总比例为39.78%。[结论]鸡胚发育过程,DNA甲基化随着胚胎的发育呈升高趋势。
关键词(KeyWords)： 甲基敏感扩增多态性;表观遗传;鸡胚;检测
基金项目(Foundation):
作者(Author): 班谦;赵宗胜;曹体婷;
Email:
DOI: 10.13989/j.cnki.0517-6611.2009.21.127
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