班谦;赵宗胜;曹体婷;

• 1:新疆石河子大学动物科技学院

摘要(Abstract)：

[目的]检测鸡胚不同发育阶段时的DNA甲基化水平。[方法]以不同发育阶段的家鸡胚胎为试验材料,提取基因组DNA,采用甲基敏感扩增片段多态性(methylation sensitive amplified polymorphism,MSAP)技术对其甲基化水平进行了初步检测。[结果]共发现甲基化位点880个,CCGG位点单链外侧的胞嘧啶甲基化位点比例为20.48%,双链CCGG位点的内侧胞嘧啶甲基化19.30%,两者总比例为39.78%。[结论]鸡胚发育过程,DNA甲基化随着胚胎的发育呈升高趋势。

关键词(KeyWords)： 甲基敏感扩增多态性;表观遗传;鸡胚;检测

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 班谦;赵宗胜;曹体婷;

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参考文献(References)：

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