胡云;徐如宏;程剑平;

• 1:贵州大学麦作研究所
• 2:贵州大学麦作研究所
• 3:贵州大学麦作研究所 贵州贵阳550025
• 4:贵州贵阳550025

摘要(Abstract)：

对来自以色列的78份野生二粒小麦进行SDS-PAGE检测,发现其高分子谷蛋白亚基具有较高的遗传多样性。在Glu-A1和Glu-B1 2个位点共有22种可确定的亚基变异类型。其中,A位点上的亚基12、*和B位点上的亚基7+8、13+16与17+18为优质亚基(出现频率分别为7.69%、8.97%和5.13%)。同时,出现了在普通小麦中不表达的1Ay亚基和许多普通小麦所稀有或缺乏的特异亚基类型,如1*7、*+8*7、*+9、7+8*、7+9*、17+18等,而且在12份材料中出现了7种未命名的新亚基类型。

关键词(KeyWords)： 野生二粒小麦;高分子量谷蛋白亚基(HMW-GS);Glu-1;SDS-PAGE

Abstract:

Keywords:

基金项目(Foundation): 2006年人事部留学人员科技活动项目择优资助项目;; 贵州省“十一五”小麦重点攻关项目;; 贵州大学人才基金

作者(Author): 胡云;徐如宏;程剑平;

Email:

DOI:

参考文献(References)：

• [1]AVIVI L.High grain protein content in wild tetraploid wheatTriticum dicoc-caidesKorn C[C]//RAMANUJAMS,ed.Proco the 5th inter wheat genet symp.India:Vol 1.New Delhi,1979:372-380.
• [2]GRARM A,GERECHTER-AMITAI Z K,BLUM A.Wild emmer as donor ofgenes for resistance to stripe rust and for high protein content C[C]//SAKAMOTO S.Procof the 6th inter wheat genet symp.Japan:Kyoto,1983:178-192.
• [3]NEVO E,BEILES A.Pattern so resistance of Israeli wild emmer wheat topathogens.I.Predictive method by ecology and allazyme genotypes for powderymildew and leaf rust[J].Genetica,1985,67:209-222.
• [4]HUEBNER F R,DONALDSON G L,WALL J S.Wheat glutenin subunitsⅡcompositional differences[J].Cereal Chem,1974,51:240-249.
• [5]KHAN K,BUSHUKW.Studies of Glutenin.ⅩⅢ.Gel filtration,isoelectric fo-cusing,and amino acid composition studies[J].Cereal Chem,1979,56:505-512.
• [6]LAWRENCE G J,SHEPHERD K W.Chromosomal location of genes controllingseed proteins in species related to wheat[J].Theor Appl Genet,1981,59:25-31.
• [7]SHEWRY P R,MIFLIN B J.Seed storage proteins of economically importantcereals[J].Adv Cereal Sci Technol,1985,7:1-84.
• [8]WEE GELS P L,HAMER R J,SCHOFIELD J D.Functional properties ofwheat glutenin[J].J Cereal Sci,1996,23:1-18.
• [9]PAYNE P I,NIGHTINGALE MA,KRATTIGER A F.Therelation ship betweenHMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties[J].J Sci Food Agric,1987,40:51-65.
• [10]PAYNE P I.Genetics of wheat storage protein and the effect of allelic variationon bread making quality[J].Ann Rev Plant Physiol,1987,38:141-153.
• [11]HARBERD N P,FLAVELL R B,THOMPSON R D.Identification of a trans-poson-like insertion in aGlu-1 allele of wheat[J].Mol GenGenet,1987,209:326-332.
• [12]LEVY AA,FELDMANM.Location of genes for high grain protein percentageand other quantitative traits in wild wheatTriticum turgidum var1 dicoccoi des[J].Euphytica,1989,41:113-122.
• [13]LEVY A A,FELDMANM.Intra-and inter-population variation ingrain proteinpercentage in wild tetraploid wheat,Triticum turgidum var1dicoccoi des[J].Euphytica,1989,42:251-258.
• [14]PAYNE P I,LAWRENCE G J.Catalogue of alleles for the complex geneloc,Glu-A1,Glu-B1and Glu-D1 which code for high molecular weight subunits ofglutenin in hexploid wheat[J].Cereal ResComm,1983,11:29-35.
• [15]ANDERSON O D,GREENE F C.The characterization and comparative analy-sis of high molecular weight glutenin genes from genome A and B of ahexaploid bread wheat[J].Theor1 Appl1 Genet1,1989,77:689-700.
• [16]MACKIE AM,LAGUDAH E S,SHARP P J,et all.Molecular and biochemi-cal characterization of HMW glutenin subunits from T1tauschii and the DGenome of hexaploid wheat[J].Journal of Cereal Science,1996,23:213-225.
• [17]刘虹.波兰小麦(Triticum polonicumL)种子贮藏蛋白遗传多样性研究[D].雅安:四川农业大学,2005:119-221.
• [18]VALLEGA V,WAINES J G.High molecular weight glutenin subunit variationinTriticum turgidum var1dicoccum[J].Theor1 Appl1 Genet1,1987,74:706-710.
• [19]BRANLARD G,AUTRAN J C,MONNEVEUX G.High molecular glutenin sub-unit in durum wheat(T.durum)[J].Theor1 Appl1 Genet,1989,78:353-358.
• [20]PAYNE P I,HOLT L M,LAW C N.Structural and generical studies on thehigh molecular weight of wheat gluteninl Part 1:allelic variation in subunits a-mong varieties of wheat(T.aestiv um)[J].Theor1 Appl1 Genet,1981,60:229-236.
• [21]BELAY G,MERKER A,TESEMMAT.Cytogenetic studies in Ethiopian lan-draces of tetraploid wheat(Triticum turgidumL.)I.Spikemorphology vsploidy level and karyomorphology[J].Hereditas Landskrona,1994,121(1):45-52.
• [22]RAUT V M,PATIL V P,DEODIKAR G B.Genetic studies in tetraploid-wheats.VII.Inheritance of seedling resistance against stem rust races[J].Biovi-gyanam,1984,10:2,101-106.
• [23]GOL-’DENBERG Z V.Content of protein and tryptophan in some species ofwheat and rye and in wheat-rye amphidiploids[J].Bulletin of the Academy ofSciences of the Georgian SSR,1984,114:2,373-376.
• [24]余伟丽,李桂江,伍碧华,等.野生二粒小麦HWM-GS特异性及其对小麦的可利用性研究[J].四川农业大学学报,2005,23(3):253-257.
• [25]CIAFFI M,LAFIANDRA D,PORCEDDU E,et al.Storage-protein variationin wild emmer wheat(Triticum turgidumssp dicoccoides)from Jordan andTurkey.Electrophoretic characterization of genotypes[J].Theor Appl Genet,1993,86:474-480.
• [26]张利,魏凌基,韩新年,等.新疆及外引的部分小麦种质资源高分子量谷蛋白亚基分析初报[J].石河子大学学报,2004,22(1):24-26.
• [27]白建荣,贾旭,刘坤凡,等.乌拉圭小麦(Triticum urartu)1Ay高分子量谷蛋白亚基基因编码区的克隆与鉴定[J].植物学报,2004,46(4):463-467.
• [28]WAN Y,WANG D,SHEWRY P R,et al.Isolation and characterization of fivenovel high molecular weight subunit of glutenin genes fromTriticu mtimopheeviandAegilop scylindrical[J].Theor Appl Genet,2002,104(5):828-839.
• [29]庄萍萍,郭志富,颜择明,等.波斯小麦高分子量谷蛋白亚基分析[J].西南农业学报,2006,19(1):5-9.
• [30]郝晨阳,尚勋武,张海泉.甘肃省春小麦品种高分子量麦谷蛋白亚基组成分析[J].植物遗传资源学报,2004,5(1):38-42.